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Literature summary extracted from
- Single-strand-specific nuclease from the nucleoplasm of rye germ nuclei (1980), Eur. J. Biochem., 108, 285-293.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | NaCl | strong inhibition above 100 mM | Secale cereale |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.30.1 | nucleoplasm | - |
Secale cereale | 5654 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 45000 | - |
SDS-PAGE | Secale cereale |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | DNA + H2O | Secale cereale | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.30.1 | Secale cereale | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.30.1 | from nucleoplasm | Secale cereale |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | 3'-AMP + H2O | - |
Secale cereale | adenosine + phosphate | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Secale cereale | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | dsDNA + H2O | cleavage of phage PM2 superhelical DNA to relaxed, nicked-circular form and unit length linear form molecules | Secale cereale | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | polyadenylic acid + H2O | - |
Secale cereale | 5'-AMP + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | polycytosylic acid + H2O | only small degradation | Secale cereale | 5'-CMP + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | polyguanylic acid + H2O | only small degradation | Secale cereale | 5'-GMP + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | polyuridylic acid + H2O | - |
Secale cereale | 5'-UMP + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Secale cereale | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.30.1 | rye germ nuclease | - |
Secale cereale |
| 3.1.30.1 | single-strand-specific nuclease | - |
Secale cereale |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 5.4 | - |
- |
Secale cereale |
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Release 2023.1 (January 2023)
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