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Literature summary extracted from

  • Cobianchi, F.; Ciarrocchi, G.; Attolini, C.; Falaschi, A.
    Properties of the high-molecular-weight deoxyribonuclease of Bacillus subtilis degrading single-stranded DNA (1978), Eur. J. Biochem., 84, 533-539.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.30.1 Triton X-100 10fold stimulation of activity with 0.3% v/v Bacillus subtilis
3.1.30.1 Trypsin almost 2.5fold stimulation of activity, probably due to a conversion into a smaller enzyme form Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.30.1 intracellular
-
 Bacillus subtilis 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.30.1 36000 38000 SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.1 DNA + H2O Bacillus subtilis
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.1 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.30.1
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.1 DNA + H2O
-
 Bacillus subtilis 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 ssDNA + H2O
-
 Bacillus subtilis 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.30.1 Bacillus subtilis deoxyribonuclease
-
 Bacillus subtilis
3.1.30.1 DNase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.30.1 37
-
-
 Bacillus subtilis