BRENDA - Enzyme Database

Purification and characterization of a DNA single strand specific endonuclease from human cells

Wang, E.C.; Furth, J.J.; Rose, J.A.; Biochemistry 17, 544-549 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.30.1
Ca2+
35.1% of maximal activity with 0.1 mM denaturated DNA, 74.7% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Homo sapiens
3.1.30.1
Cu2+
46.0% of maximal activity with 0.1 mM denaturated DNA, 56.0% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Homo sapiens
3.1.30.1
Fe2+
47.7% of maximal activity with 0.1 mM denaturated DNA, almost no activity with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Homo sapiens
3.1.30.1
Hg2+
31.5% of maximal activity with 0.1 mM denaturated DNA, 48.2% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Homo sapiens
3.1.30.1
Mn2+
6.5% of maximal activity with 0.1 mM denaturated DNA, 8.4% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Homo sapiens
3.1.30.1
N-ethylmaleimide
with 5 mM 67% reduction of hydrolysis of polydeoxythymidylic acid
Homo sapiens
3.1.30.1
NaCl
inhibition of polydeoxythymidylic acid at 10-50 mM, not reversible by addition of Mg2+
Homo sapiens
3.1.30.1
Zn2+
33.7% of maximal activity with 0.1 mM denaturated DNA, 65.2% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.30.1
0.02
-
denaturated DNA
-
Homo sapiens
3.1.30.1
0.02
-
polydeoxythymidylic acid
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.30.1
Mg2+
less than 1% of maximal activity without Mg2+, maximal activity at 0.5 mM
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.30.1
54000
-
SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.30.1
DNA + H2O
Homo sapiens
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.30.1
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.30.1
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.30.1
KB cell
pharyngal carcinoma cell line
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.30.1
0.01
-
purified enzyme, polydeoxythymidylic acid as substrate
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.30.1
DNA + H2O
-
134996
Homo sapiens
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
3.1.30.1
dsDNA + H2O
-
134996
Homo sapiens
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
3.1.30.1
polydeoxyadenylic acid + H2O
-
134996
Homo sapiens
5'-dAMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
3.1.30.1
polydeoxycytosylic acid + H2O
only little degradation
134996
Homo sapiens
5'-dCMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
3.1.30.1
polydeoxyguanylic acid + H2O
-
134996
Homo sapiens
5'-dGMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
3.1.30.1
polydeoxythymidylic acid + H2O
-
134996
Homo sapiens
5'-dTMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
3.1.30.1
polyuridylic acid + H2O
80% of activity with polydeoxythymidylic acid as substrate
134996
Homo sapiens
5'-UMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
3.1.30.1
ssDNA + H2O
-
134996
Homo sapiens
5'-phosphomononucleotides + 5'-phosphooligonucleotides
no detectable 5'-phospho mononucleotides, most 5'-phospho tetranucleotides
134996
Homo sapiens
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.30.1
37
-
-
Homo sapiens
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.30.1
50
-
40% loss of activity with polyuridylic acid or polydeoxythymidylic acid as substrate after heating of the enzyme fraction for 5 min
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.30.1
9.2
-
with denaturated DNA and polydeoxythymidylic acid as substrate
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.30.1
4.5
10.5
12.3% of maximal activity at pH 4.5, 43.3% at pH 10.5
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.30.1
Ca2+
35.1% of maximal activity with 0.1 mM denaturated DNA, 74.7% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Homo sapiens
3.1.30.1
Cu2+
46.0% of maximal activity with 0.1 mM denaturated DNA, 56.0% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Homo sapiens
3.1.30.1
Fe2+
47.7% of maximal activity with 0.1 mM denaturated DNA, almost no activity with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Homo sapiens
3.1.30.1
Hg2+
31.5% of maximal activity with 0.1 mM denaturated DNA, 48.2% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Homo sapiens
3.1.30.1
Mn2+
6.5% of maximal activity with 0.1 mM denaturated DNA, 8.4% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Homo sapiens
3.1.30.1
N-ethylmaleimide
with 5 mM 67% reduction of hydrolysis of polydeoxythymidylic acid
Homo sapiens
3.1.30.1
NaCl
inhibition of polydeoxythymidylic acid at 10-50 mM, not reversible by addition of Mg2+
Homo sapiens
3.1.30.1
Zn2+
33.7% of maximal activity with 0.1 mM denaturated DNA, 65.2% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.30.1
0.02
-
denaturated DNA
-
Homo sapiens
3.1.30.1
0.02
-
polydeoxythymidylic acid
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.30.1
Mg2+
less than 1% of maximal activity without Mg2+, maximal activity at 0.5 mM
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.30.1
54000
-
SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.30.1
DNA + H2O
Homo sapiens
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
Homo sapiens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.30.1
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.30.1
KB cell
pharyngal carcinoma cell line
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.30.1
0.01
-
purified enzyme, polydeoxythymidylic acid as substrate
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.30.1
DNA + H2O
-
134996
Homo sapiens
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
3.1.30.1
dsDNA + H2O
-
134996
Homo sapiens
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
3.1.30.1
polydeoxyadenylic acid + H2O
-
134996
Homo sapiens
5'-dAMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
3.1.30.1
polydeoxycytosylic acid + H2O
only little degradation
134996
Homo sapiens
5'-dCMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
3.1.30.1
polydeoxyguanylic acid + H2O
-
134996
Homo sapiens
5'-dGMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
3.1.30.1
polydeoxythymidylic acid + H2O
-
134996
Homo sapiens
5'-dTMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
3.1.30.1
polyuridylic acid + H2O
80% of activity with polydeoxythymidylic acid as substrate
134996
Homo sapiens
5'-UMP + 5'-phosphooligonucleotides
-
134996
Homo sapiens
?
3.1.30.1
ssDNA + H2O
-
134996
Homo sapiens
5'-phosphomononucleotides + 5'-phosphooligonucleotides
no detectable 5'-phospho mononucleotides, most 5'-phospho tetranucleotides
134996
Homo sapiens
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.30.1
37
-
-
Homo sapiens
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.30.1
50
-
40% loss of activity with polyuridylic acid or polydeoxythymidylic acid as substrate after heating of the enzyme fraction for 5 min
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.30.1
9.2
-
with denaturated DNA and polydeoxythymidylic acid as substrate
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.30.1
4.5
10.5
12.3% of maximal activity at pH 4.5, 43.3% at pH 10.5
Homo sapiens