EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.30.1 | Ca2+ | 35.1% of maximal activity with 0.1 mM denaturated DNA, 74.7% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition | Homo sapiens | |
3.1.30.1 | Cu2+ | 46.0% of maximal activity with 0.1 mM denaturated DNA, 56.0% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition | Homo sapiens | |
3.1.30.1 | Fe2+ | 47.7% of maximal activity with 0.1 mM denaturated DNA, almost no activity with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition | Homo sapiens | |
3.1.30.1 | Hg2+ | 31.5% of maximal activity with 0.1 mM denaturated DNA, 48.2% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition | Homo sapiens | |
3.1.30.1 | Mn2+ | 6.5% of maximal activity with 0.1 mM denaturated DNA, 8.4% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition | Homo sapiens | |
3.1.30.1 | N-ethylmaleimide | with 5 mM 67% reduction of hydrolysis of polydeoxythymidylic acid | Homo sapiens | |
3.1.30.1 | NaCl | inhibition of polydeoxythymidylic acid at 10-50 mM, not reversible by addition of Mg2+ | Homo sapiens | |
3.1.30.1 | Zn2+ | 33.7% of maximal activity with 0.1 mM denaturated DNA, 65.2% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.30.1 | 0.02 | - |
denaturated DNA | - |
Homo sapiens | |
3.1.30.1 | 0.02 | - |
polydeoxythymidylic acid | - |
Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.30.1 | Mg2+ | less than 1% of maximal activity without Mg2+, maximal activity at 0.5 mM | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.30.1 | 54000 | - |
SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.30.1 | DNA + H2O | Homo sapiens | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.30.1 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.30.1 | - |
Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.30.1 | KB cell | pharyngal carcinoma cell line | Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.30.1 | 0.01 | - |
purified enzyme, polydeoxythymidylic acid as substrate | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.30.1 | denatured DNA + H2O | - |
Homo sapiens | ? | - |
? | |
3.1.30.1 | DNA + H2O | - |
Homo sapiens | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
3.1.30.1 | dsDNA + H2O | - |
Homo sapiens | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
3.1.30.1 | polydeoxyadenylic acid + H2O | - |
Homo sapiens | 5'-dAMP + 5'-phosphooligonucleotides | - |
? | |
3.1.30.1 | polydeoxycytosylic acid + H2O | only little degradation | Homo sapiens | 5'-dCMP + 5'-phosphooligonucleotides | - |
? | |
3.1.30.1 | polydeoxyguanylic acid + H2O | - |
Homo sapiens | 5'-dGMP + 5'-phosphooligonucleotides | - |
? | |
3.1.30.1 | polydeoxythymidylic acid + H2O | - |
Homo sapiens | 5'-dTMP + 5'-phosphooligonucleotides | - |
? | |
3.1.30.1 | polyuridylic acid + H2O | 80% of activity with polydeoxythymidylic acid as substrate | Homo sapiens | 5'-UMP + 5'-phosphooligonucleotides | - |
? | |
3.1.30.1 | ssDNA + H2O | - |
Homo sapiens | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | no detectable 5'-phospho mononucleotides, most 5'-phospho tetranucleotides | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.30.1 | KB endonuclease | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.30.1 | 37 | - |
- |
Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.30.1 | 50 | - |
40% loss of activity with polyuridylic acid or polydeoxythymidylic acid as substrate after heating of the enzyme fraction for 5 min | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.30.1 | 9.2 | - |
with denaturated DNA and polydeoxythymidylic acid as substrate | Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.30.1 | 4.5 | 10.5 | 12.3% of maximal activity at pH 4.5, 43.3% at pH 10.5 | Homo sapiens |