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Literature summary extracted from

  • Martin, S.A.; Ullrich, R.C.; Meyer, W.L.
    Partial purification and properties of a nuclease from Schizophyllum commune with a preference toward single-stranded nucleic acid (1986), Biochim. Biophys. Acta, 867, 67-75.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.1 8-hydroxychinoline 5-sulfonate 50% of activity towards ssDNA with 0.005 mM Schizophyllum commune
3.1.30.1 citrate at 0.2 M 50% of initial activity with ssDNA Schizophyllum commune
3.1.30.1 EDTA 50% of activity towards ssDNA with 0.0003 mM Schizophyllum commune
3.1.30.1 F- at 0.2 M 30% of initial activity with ssDNA Schizophyllum commune
3.1.30.1 heparin
-
 Schizophyllum commune
3.1.30.1 Ionic strength inhibition of activity at ionic strength above 0.3 M Schizophyllum commune
3.1.30.1 phosphate
-
 Schizophyllum commune
3.1.30.1 Polyvinyl sulfate
-
 Schizophyllum commune
3.1.30.1 sodium bisphosphate at 0.2 M 10% of initial activity with ssDNA Schizophyllum commune
3.1.30.1 Zn2+ 54% of initial activity towards ssDNA with 0.1 mM Zn2+; almost complete inactivation at 1.0 mM Schizophyllum commune

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.1 Co2+ at 0.0014 mM 105% restoration of activity after EDTA-inactivation Schizophyllum commune
3.1.30.1 Mn2+ at 0.0014 mM 53% restoration of activity after EDTA-inactivation Schizophyllum commune
3.1.30.1 Zn2+ stimulation of activity towards ssDNA at 0.001-0.01 mM, at 0.0014 mM 103% restoration of activity after EDTA-inactivation Schizophyllum commune

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.30.1 34000
-
-
 Schizophyllum commune

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.1 DNA + H2O Schizophyllum commune
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 RNA + H2O Schizophyllum commune
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.1 Schizophyllum commune
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.30.1
-
 Schizophyllum commune

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.1 DNA + H2O
-
 Schizophyllum commune 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 dsDNA + H2O
-
 Schizophyllum commune 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 RNA + H2O
-
 Schizophyllum commune 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 ssDNA + H2O
-
 Schizophyllum commune 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.30.1 SC nuclease
-
 Schizophyllum commune

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.30.1 32
-
-
 Schizophyllum commune

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.30.1 30 70 50% of activity with ssDNA at 47°C, with dsDNA at 49°C Schizophyllum commune

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.30.1 6.5 7.5 50% of optimal activity towards ssDNA and dsDNA Schizophyllum commune

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.30.1 5.5 9 in 30 mM buffer with 200 mM NaCl, more stable above pH 7.0 than below Schizophyllum commune