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Literature summary extracted from
- Purification and properties of the mung bean nuclease (1980), Methods Enzymol., 65, 263-276.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | L-serine | - |
Vigna radiata var. radiata |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | Zn2+ | at 1 mM | Vigna radiata var. radiata | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 39000 | - |
reduction with 2-mercaptoethanol prior to SDS-PAGE generates two polypeptides of 25000 and 15000, linked covalently by disulfide bond, intact and cleaved forms are equally active | Vigna radiata var. radiata |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | DNA + H2O | Vigna radiata var. radiata | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.30.1 | Vigna radiata var. radiata | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.30.1 | glycoprotein | 29% carbohydrate by weight | Vigna radiata var. radiata |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.30.1 | - |
Vigna radiata var. radiata |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.30.1 | -20°C, 50% glycerol v/v, 6 months, no loss of activity of the purified enzyme | Vigna radiata var. radiata |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | 3'-AMP + H2O | - |
Vigna radiata var. radiata | adenosine + phosphate | - |
? | |
| 3.1.30.1 | DNA + H2O | - |
Vigna radiata var. radiata | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | dsDNA + H2O | - |
Vigna radiata var. radiata | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | after introduction of nicks and double-strand scissions liberation of 5'-phosphomononucleotides and 5'-phosphooligonucleotides from the newly created termini | ? | |
| 3.1.30.1 | ssDNA + H2O | - |
Vigna radiata var. radiata | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.30.1 | mung bean nuclease | - |
Vigna radiata var. radiata |
| 3.1.30.1 | mung bean nuclease I | - |
Vigna radiata var. radiata |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 7 | 8 | stable without additions | Vigna radiata var. radiata |
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Release 2023.1 (January 2023)
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