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Literature summary extracted from

  • Fraser, M.J.
    Purification and properties of Neurospora crassa endo-exonuclease, an enzyme which can be converted to a single-strand specific endonuclease (1980), Methods Enzymol., 65, 255-263.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.1 Mg2+ about 40% inhibition of hydrolysis of RNA with 10 mM Mg2+ Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.1 DNA + H2O Neurospora crassa
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 RNA + H2O Neurospora crassa
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.1 Neurospora crassa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.30.1 conidium
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.30.1 810
-
 with heat-denaturated DNA at pH 8.0 in the presence of 10 mM Mg2+ Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.1 DNA + H2O
-
 Neurospora crassa 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 RNA + H2O
-
 Neurospora crassa 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 ssDNA + H2O
-
 Neurospora crassa 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.1 7.5 8.5 0.1-0.2 M Tris-HCl buffer Neurospora crassa