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Literature summary extracted from
- Immobilization of single-strand specific nuclease (S1 nuclease) from Aspergillus oryzae (1987), Appl. Biochem. Biotechnol., 14, 231-240.
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | DNA + H2O | Aspergillus oryzae | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.30.1 | Aspergillus oryzae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.30.1 | - |
Aspergillus oryzae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | DNA + H2O | - |
Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.30.1 | S1 nuclease | - |
Aspergillus oryzae |
| 3.1.30.1 | single-strand-specific nuclease | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 37 | - |
- |
Aspergillus oryzae |
| 3.1.30.1 | 55 | - |
soluble enzyme | Aspergillus oryzae |
| 3.1.30.1 | 65 | - |
immobilized enzyme | Aspergillus oryzae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 60 | 80 | at 70°C complete loss of activity of the soluble enzyme, 40% of maximal activity of the immobilized enzyme at this temperature, 20% at 80°C | Aspergillus oryzae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 3.8 | 4.3 | pH 3.8 for the immobilized, pH 4.3 for the soluble enzyme | Aspergillus oryzae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 3.6 | 5.2 | comparatively higher stability of the immobilized than the soluble enzyme | Aspergillus oryzae |
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Release 2023.1 (January 2023)
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