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Literature summary extracted from

  • Oleson, A.E.; Hoganson, E.D.
    S1 nuclease of Aspergillus oryzae: characterization of the associated phosphomonoesterase activity (1981), Arch. Biochem. Biophys., 211, 478-484.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.1 5'-ADP
-
 Aspergillus oryzae
3.1.30.1 5'-AMP
-
 Aspergillus oryzae
3.1.30.1 5'-dADP
-
 Aspergillus oryzae
3.1.30.1 5'-dAMP
-
 Aspergillus oryzae
3.1.30.1 5'-dATP
-
 Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.30.1 3.4
-
 adenosine 3',5'-bisphosphate
-
 Aspergillus oryzae
3.1.30.1 37.6
-
 3'-AMP
-
 Aspergillus oryzae
3.1.30.1 47.1
-
 3'-GMP
-
 Aspergillus oryzae
3.1.30.1 69.9
-
 deoxythymidine 3',5'-bisphosphate
-
 Aspergillus oryzae
3.1.30.1 71.2
-
 3'-CMP
-
 Aspergillus oryzae
3.1.30.1 79.3
-
 3'-UMP
-
 Aspergillus oryzae
3.1.30.1 92
-
 3'-dAMP
-
 Aspergillus oryzae
3.1.30.1 208
-
 2'-AMP
-
 Aspergillus oryzae
3.1.30.1 223
-
 3'-dGMP
-
 Aspergillus oryzae
3.1.30.1 470
-
 3'-dCMP
-
 Aspergillus oryzae
3.1.30.1 608
-
 3'-dTMP
-
 Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.1 DNA + H2O Aspergillus oryzae
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.1 Aspergillus oryzae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.30.1 0.63
-
 with 3'-dAMP as substrate Aspergillus oryzae
3.1.30.1 1.06
-
 with 2'-AMP as substrate Aspergillus oryzae
3.1.30.1 2.39
-
 with 3'-dGMP as substrate Aspergillus oryzae
3.1.30.1 3.76
-
 with 3'dCMP as substrate Aspergillus oryzae
3.1.30.1 4.83
-
 with 3'-dTMP as substrate Aspergillus oryzae
3.1.30.1 17.2
-
 with deoxythymidine 3',5'-bisphosphate as substrate Aspergillus oryzae
3.1.30.1 241
-
 with 3'-GMP as substrate Aspergillus oryzae
3.1.30.1 242
-
 with adenosine 3',5'-bisphosphate as substrate Aspergillus oryzae
3.1.30.1 248
-
 with 3'-AMP as substrate Aspergillus oryzae
3.1.30.1 255
-
 with 3'-UMP as substrate Aspergillus oryzae
3.1.30.1 266
-
 with 3'-CMP as substrate Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.1 2'-AMP + H2O
-
 Aspergillus oryzae adenosine + phosphate
-
 ?
3.1.30.1 3'-AMP + H2O
-
 Aspergillus oryzae adenosine + phosphate
-
 ?
3.1.30.1 3'-CMP + H2O
-
 Aspergillus oryzae cytosine + phosphate
-
 ?
3.1.30.1 3'-dAMP + H2O
-
 Aspergillus oryzae 3'-deoxyadenosine + phosphate
-
 ?
3.1.30.1 3'-dCMP + H2O
-
 Aspergillus oryzae deoxycytosine + phosphate
-
 ?
3.1.30.1 3'-dGMP + H2O
-
 Aspergillus oryzae deoxyguanosine + phosphate
-
 ?
3.1.30.1 3'-dTMP + H2O
-
 Aspergillus oryzae deoxythymidine + phosphate
-
 ?
3.1.30.1 3'-GMP + H2O
-
 Aspergillus oryzae guanosine + phosphate
-
 ?
3.1.30.1 3'-UMP + H2O
-
 Aspergillus oryzae uridine + phosphate
-
 ?
3.1.30.1 adenosine 3',5'-bisphosphate + H2O
-
 Aspergillus oryzae 5'-AMP + phosphate
-
 ?
3.1.30.1 adenosine 3'-diphosphate 5'-phosphate
-
 Aspergillus oryzae 5'-AMP + diphosphate
-
 ?
3.1.30.1 ApA + H2O
-
 Aspergillus oryzae adenosine + AMP
-
 ?
3.1.30.1 deoxythymidine 3',5'-bisphosphate
-
 Aspergillus oryzae 5'-dTMP + phosphate
-
 ?
3.1.30.1 DNA + H2O
-
 Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.30.1 S1 nuclease
-
 Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.1 4.5
-
-
 Aspergillus oryzae