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Literature summary extracted from

  • Lehman, R.I.
    Endonucleases specific for single-stranded polynucleotides (1981), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 193-201.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.30.1 analysis
-
Vigna radiata var. radiata
3.1.30.1 analysis
-
Neurospora crassa
3.1.30.1 analysis in DNA-RNA annaeling experiments as reagent for selective removal of nonhybridized polynucleotides Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.1 5'-ATP 50% inhibition by 0.5 mM Neurospora crassa
3.1.30.1 EDTA
-
Aspergillus oryzae
3.1.30.1 EDTA 95% inhibition at 0.1 mM, reversed by equivalent amounts of Co2+ Neurospora crassa
3.1.30.1 EDTA no activity after dialysis with 1 mM, no restoration by addition of Zn2+, Co2+, Mg2+, Mn2+, Ca2+ or cysteine Vigna radiata var. radiata
3.1.30.1 NaCl optimal activity at 100 mM, 55% of maximal rate at 400 mM Aspergillus oryzae
3.1.30.1 phosphate
-
Aspergillus oryzae
3.1.30.1 phosphate 80% inhibition by 33 mM potassium phosphate, pH 7.5 Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.1 Co2+
-
Neurospora crassa
3.1.30.1 Co2+
-
Aspergillus oryzae
3.1.30.1 Zn2+
-
Vigna radiata var. radiata
3.1.30.1 Zn2+
-
Neurospora crassa
3.1.30.1 Zn2+
-
Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.30.1 32000
-
-
Aspergillus oryzae
3.1.30.1 39000
-
reduction with 2-mercaptoethanol prior to SDS-PAGE generates two polypeptides of 25000 and 15000, linked covalently by disulfide bond, intact and cleaved forms are equally active Vigna radiata var. radiata
3.1.30.1 42000
-
gel filtration Vigna radiata var. radiata
3.1.30.1 43000
-
sucrose density gradient sedimentation Vigna radiata var. radiata
3.1.30.1 55000
-
-
Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.1 DNA + H2O Vigna radiata var. radiata
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
3.1.30.1 DNA + H2O Aspergillus oryzae functional role in recombination and DNA repair in vivo 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
3.1.30.1 DNA + H2O Neurospora crassa native DNA hydrolysis probably by contaminating nuclease, its inactivation possible at 55°C 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
3.1.30.1 RNA + H2O Vigna radiata var. radiata
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
3.1.30.1 RNA + H2O Neurospora crassa
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
3.1.30.1 RNA + H2O Aspergillus oryzae 5fold more activity on DNA than RNA 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.1 Aspergillus oryzae
-
-
-
3.1.30.1 Neurospora crassa
-
-
-
3.1.30.1 Vigna radiata var. radiata
-
mung bean
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.30.1 glycoprotein
-
Aspergillus oryzae
3.1.30.1 glycoprotein 29% carbohydrate by weight Vigna radiata var. radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.30.1 conidium
-
Neurospora crassa
-
3.1.30.1 mycelium stationary phase Neurospora crassa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.1 DNA + H2O
-
Vigna radiata var. radiata 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
3.1.30.1 DNA + H2O functional role in recombination and DNA repair in vivo Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
3.1.30.1 DNA + H2O native DNA hydrolysis probably by contaminating nuclease, its inactivation possible at 55°C Neurospora crassa 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
3.1.30.1 RNA + H2O
-
Vigna radiata var. radiata 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
3.1.30.1 RNA + H2O
-
Neurospora crassa 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
3.1.30.1 RNA + H2O
-
Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
3.1.30.1 RNA + H2O
-
Neurospora crassa 5'-phosphomononucleotides + 5'-phosphooligonucleotides mainly 5'-phosphodi- and trinucleotides, at long incubation time more than 90% 5'-phosphomononucleotides ?
3.1.30.1 RNA + H2O 5fold more activity on DNA than RNA Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
3.1.30.1 ssDNA + H2O
-
Vigna radiata var. radiata 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
3.1.30.1 ssDNA + H2O
-
Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
3.1.30.1 ssDNA + H2O
-
Neurospora crassa 5'-phosphomononucleotides + 5'-phosphooligonucleotides mainly 5'-phosphodi- and trinucleotides, at long incubation time more than 90% 5'-phosphomononucleotides ?
3.1.30.1 tRNA + H2O
-
Neurospora crassa 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
3.1.30.1 tRNA + H2O hydrolysis of nonhydrogen-bonded loops Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.30.1 mung bean endonuclease
-
Vigna radiata var. radiata
3.1.30.1 S1 nuclease
-
Aspergillus oryzae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.30.1 25 65
-
Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.1 4 4.3
-
Aspergillus oryzae
3.1.30.1 5
-
because of rapid inactivation, stabilization by 0.1 mM Zn2+ or 1 mM cysteine, glutathione or dithiothreitol recommended Vigna radiata var. radiata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.30.1 6 9
-
Neurospora crassa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.30.1 0.5
-
5'-ATP
-
Neurospora crassa