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Literature summary extracted from

  • Nishizawa, A.N.; Buchanan, B.B.
    Enzyme regulation in C4 photosynthesis. Purification and properties of thioredoxin-linked fructose bisphosphatase and sedoheptulose bisphosphatase from corn leaves (1981), J. Biol. Chem., 256, 6119-6126.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.11 Thioredoxin f activates Zea mays
3.1.3.37 Dithiol absolute requirement for a dithiol at pH values below pH 7.7, i.e. reduced thioredoxin f or dithiothreitol Zea mays
3.1.3.37 Thioredoxin f activates Zea mays

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.11
-
Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 dehydroascorbate deactivates the enzyme activated by reduced thioredoxin f Zea mays
3.1.3.11 oxidized glutathione deactivates the enzyme activated by reduced thioredoxin f Zea mays
3.1.3.37 dehydroascorbate
-
Zea mays
3.1.3.37 oxidized glutathione
-
Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 Mg2+ required Zea mays
3.1.3.37 Mg2+ required Zea mays
3.1.3.37 Mg2+ no activation in absence of a dithiol reductant Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 46000
-
4 * 46000, SDS-PAGE Zea mays
3.1.3.11 184000
-
gel filtration Zea mays
3.1.3.37 38000
-
2 * 38000, SDS-PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Zea mays
-
-
-
3.1.3.37 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11
-
Zea mays
3.1.3.37
-
Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.11 leaf
-
Zea mays
-
3.1.3.37 leaf
-
Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.11 62.5
-
-
Zea mays
3.1.3.37 28.6
-
-
Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-diphosphate + H2O
-
Zea mays D-fructose 6-phosphate + phosphate
-
?
3.1.3.11 sedoheptulose 1,7-bisphosphate + H2O no activity Zea mays sedoheptulose 7-phosphate + phosphate
-
?
3.1.3.37 sedoheptulose 1,7-bisphosphate + H2O specific for Zea mays sedoheptulose 7-phosphate + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.11 tetramer 4 * 46000, SDS-PAGE Zea mays
3.1.3.37 dimer 2 * 38000, SDS-PAGE Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.11 7.7
-
in presence of thioredoxin f Zea mays
3.1.3.11 8.2
-
in absence of thioredoxin f Zea mays