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Literature summary extracted from

  • Waymack, P.P.; van Etten, R.L.
    Isolation and characterization of a homogeneous isoenzyme of wheat germ acid phosphatase (1991), Arch. Biochem. Biophys., 288, 621-633.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.3.2 50% loss of activity after lyophilization Triticum aestivum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Ag+
-
 Triticum aestivum
3.1.3.2 Cu2+
-
 Triticum aestivum
3.1.3.2 Hg2+
-
 Triticum aestivum
3.1.3.2 molybdate
-
 Triticum aestivum
3.1.3.2 additional information substrate inhibition with some substrates below pH 6 Triticum aestivum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Co2+ slight stimulation Triticum aestivum
3.1.3.2 Mg2+ slight stimulation Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 58000
-
 and a second isoenzyme with 60000 Da, gel filtration Triticum aestivum
3.1.3.2 60000
-
 and a second isoenzyme with 58000 Da, gel filtration Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Triticum aestivum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 no modification does not contain carbohydrate chains with significant amounts of terminal mannose or galactose residues Triticum aestivum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
 Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 germ
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 605
-
-
 Triticum aestivum

Storage Stability

EC Number Storage Stability Organism
3.1.3.2 4°C, pH 4-7, protein concentration 1 mg/ml, half-life of partially purified enzyme is 3 months Triticum aestivum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 22
-
 room temperature, half-life: 3-4 days Triticum aestivum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.2 3 7.5 25°C, dramatic loss of activity above pH 7.5 and below pH 3.0 Triticum aestivum
3.1.3.2 4 7 maximal stability Triticum aestivum
3.1.3.2 8
-
 25°C, half-life: 40 min Triticum aestivum