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Literature summary extracted from

  • Shimada, Y.; Shinmyo, A.; Enatsu, T.
    Purification and properties of one component of acid phosphatase produced by Aspergillus niger (1977), Biochim. Biophys. Acta, 480, 417-427.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 2-hydroyxy-5-nitrobenzyl bromide
-
Aspergillus niger
3.1.3.2 arsenate
-
Aspergillus niger
3.1.3.2 Borate
-
Aspergillus niger
3.1.3.2 Bromosuccinimide
-
Aspergillus niger
3.1.3.2 F-
-
Aspergillus niger
3.1.3.2 Fe3+ 8 mM, 45% inhibition Aspergillus niger
3.1.3.2 molybdate
-
Aspergillus niger
3.1.3.2 phosphate
-
Aspergillus niger
3.1.3.2 Tartrate inhibition by L(+)-tartrate, D(-) tartrate has no effect Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 89000
-
x * 89000, SDS-PAGE Aspergillus niger
3.1.3.2 310000
-
gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 glycoprotein contains 29% carbohydrate consisting of glucosamine, mannose and galactose Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 mycelium
-
Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 additional information
-
-
Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
3.1.3.2 -15°C, pH 4.6, 0.05 M citrate buffer, stable for at least 3 months Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-naphthyl phosphate + H2O 86% of the activity with p-nitrophenyl phosphate Aspergillus niger 1-naphthol + phosphate
-
?
3.1.3.2 2-glycerophosphate + H2O 72% of the activity with p-nitrophenyl phosphate Aspergillus niger glycerol + phosphate
-
?
3.1.3.2 5'-ADP + H2O 14% of the activity with p-nitrophenyl phosphate Aspergillus niger AMP + phosphate
-
?
3.1.3.2 5'-AMP + H2O 105% of the activity with p-nitrophenyl phosphate Aspergillus niger adenosine + phosphate
-
?
3.1.3.2 5'-CMP + H2O 97% of the activity with p-nitrophenyl phosphate Aspergillus niger cytidine + phosphate
-
?
3.1.3.2 5'-GMP + H2O 33% of the activity with p-nitrophenyl phosphate Aspergillus niger guanosine + phosphate
-
?
3.1.3.2 6-phosphogluconate + H2O 17% of the activity with p-nitrophenyl phosphate Aspergillus niger gluconate + phosphate
-
?
3.1.3.2 alpha-glycerophosphate + H2O 38% of the activity with p-nitrophenyl phosphate Aspergillus niger glycerol + phosphate
-
?
3.1.3.2 bis(p-nitrophenyl) phosphate + H2O 2% of the activity with p-nitrophenyl phosphate Aspergillus niger ?
-
?
3.1.3.2 D-fructose 1,6-diphosphate + H2O 4% of the activity with p-nitrophenyl phosphate Aspergillus niger ?
-
?
3.1.3.2 D-fructose 6-phosphate + H2O 32% of the activity with p-nitrophenyl phosphate Aspergillus niger D-fructose + phosphate
-
?
3.1.3.2 D-ribose 5-phosphate + H2O 36% of the activity with p-nitrophenyl phosphate Aspergillus niger D-ribose + phosphate
-
?
3.1.3.2 glucose 1-phosphate + H2O 45% of the activity with p-nitrophenyl phosphate Aspergillus niger glucose + phosphate
-
?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
Aspergillus niger p-nitrophenol + phosphate
-
?
3.1.3.2 phosphopyruvate + H2O 40% of the activity with p-nitrophenyl phosphate Aspergillus niger pyruvate + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 ? x * 89000, SDS-PAGE Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 4
-
hydrolysis of p-nitrophenyl phosphate Aspergillus niger