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Literature summary extracted from

  • Rengasamy, A.; Selvam, R.; Gnanam, A.
    Isolation and properties of an acid phosphatase from thylakoid membranes of Sorghum vulgare (1981), Arch. Biochem. Biophys., 209, 230-236.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.3.2 5 M urea, 2 h, 71% of the initial activity is retained Sorghum bicolor
3.1.3.2 denaturation by guanidine hydrochloride and SDS Sorghum bicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 ATP competitive inhibitor of hydrolysis of p-nitrophenyl phosphate Sorghum bicolor
3.1.3.2 F-
-
 Sorghum bicolor
3.1.3.2 Hg2+ 5 mM HgCl2, 82% inhibition Sorghum bicolor
3.1.3.2 KH2PO4
-
 Sorghum bicolor
3.1.3.2 molybdate
-
 Sorghum bicolor
3.1.3.2 Pb(CH3COO)2
-
 Sorghum bicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.3
-
 p-nitrophenyl phosphate
-
 Sorghum bicolor
3.1.3.2 0.3
-
 Phenyl phosphate
-
 Sorghum bicolor

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.2 thylakoid
-
 Sorghum bicolor 9579
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 26000
-
 1 * 26000, SDS-PAGE Sorghum bicolor
3.1.3.2 27000
-
 gel filtration Sorghum bicolor

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Sorghum bicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
 Sorghum bicolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 leaf
-
 Sorghum bicolor
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-glycerate phosphate + H2O
-
 Sorghum bicolor glycerate + phosphate
-
 ?
3.1.3.2 2-naphthyl phosphate + H2O
-
 Sorghum bicolor 2-naphthol + phosphate
-
 ?
3.1.3.2 5'-ADP + H2O
-
 Sorghum bicolor AMP + phosphate
-
 ?
3.1.3.2 5'-GMP + H2O
-
 Sorghum bicolor guanosine + phosphate
-
 ?
3.1.3.2 ATP + H2O
-
 Sorghum bicolor ADP + phosphate
-
 ?
3.1.3.2 creatine phosphate + H2O
-
 Sorghum bicolor creatine + phosphate
-
 ?
3.1.3.2 D-fructose 1,6-diphosphate + H2O
-
 Sorghum bicolor ?
-
 ?
3.1.3.2 D-fructose 6-phosphate + H2O
-
 Sorghum bicolor D-fructose + phosphate
-
 ?
3.1.3.2 D-ribose 5-phosphate + H2O
-
 Sorghum bicolor D-ribose + phosphate
-
 ?
3.1.3.2 diphosphate + H2O
-
 Sorghum bicolor 2 phosphate
-
 ?
3.1.3.2 glucose 1-phosphate + H2O
-
 Sorghum bicolor glucose + phosphate
-
 ?
3.1.3.2 Glucose 6-phosphate + H2O
-
 Sorghum bicolor Glucose + phosphate
-
 ?
3.1.3.2 GTP + H2O
-
 Sorghum bicolor GDP + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Sorghum bicolor p-nitrophenol + phosphate
-
 ?
3.1.3.2 phenyl phosphate + H2O
-
 Sorghum bicolor phenol + phosphate
-
 ?
3.1.3.2 phosphopyruvate + H2O
-
 Sorghum bicolor pyruvate + phosphate
-
 ?
3.1.3.2 UTP + 2 H2O
-
 Sorghum bicolor UMP + 2 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 monomer 1 * 26000, SDS-PAGE Sorghum bicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 50
-
 30 min, 30% loss of activity Sorghum bicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5
-
-
 Sorghum bicolor