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Literature summary extracted from

  • Hayman, A.R.; Warburton, M.J.; Pringle, J.A.S.; Coles, B.; Chambers, T.J.
    Purification and characterization of a tartrate-resistant acid phosphatase from human osteoclastomas (1989), Biochem. J., 261, 601-609.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 2-mercaptoethanol
-
 Homo sapiens
3.1.3.2 ascorbate
-
 Homo sapiens
3.1.3.2 Cupric sulfate
-
 Homo sapiens
3.1.3.2 EDTA
-
 Homo sapiens
3.1.3.2 F- NaF Homo sapiens
3.1.3.2 Ferrous ammonium sulfate
-
 Homo sapiens
3.1.3.2 molybdate
-
 Homo sapiens
3.1.3.2 Sodium chloroaurate
-
 Homo sapiens
3.1.3.2 Sodium dithionite
-
 Homo sapiens
3.1.3.2 Tartrate tartrate has no effect Homo sapiens
3.1.3.2 Zn2+
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.26
-
 GTP
-
 Homo sapiens
3.1.3.2 0.265
-
 ATP
-
 Homo sapiens
3.1.3.2 0.276
-
 Phenolphthalein diphosphate
-
 Homo sapiens
3.1.3.2 0.284
-
 4-methylumbelliferyl phosphate
-
 Homo sapiens
3.1.3.2 0.299
-
 TTP
-
 Homo sapiens
3.1.3.2 0.333
-
 ADP
-
 Homo sapiens
3.1.3.2 0.339
-
 phosphoenolpyruvate
-
 Homo sapiens
3.1.3.2 0.348
-
 GDP
-
 Homo sapiens
3.1.3.2 0.377
-
 CTP
-
 Homo sapiens
3.1.3.2 0.476
-
 TDP
-
 Homo sapiens
3.1.3.2 0.606
-
 CDP
-
 Homo sapiens
3.1.3.2 0.606
-
 Phenyl phosphate
-
 Homo sapiens
3.1.3.2 0.615
-
 UDP
-
 Homo sapiens
3.1.3.2 0.714
-
 UTP
-
 Homo sapiens
3.1.3.2 0.909
-
 Alpha-naphthyl phosphate
-
 Homo sapiens
3.1.3.2 1
-
 p-nitrophenyl phosphate
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Fe2+ enzyme contains 4.8 mol Fe2+/3+ per mol of enzyme Homo sapiens
3.1.3.2 Mg2+ activates, enzyme contains 1.7 mol of Mg2+ per mol of enzyme Homo sapiens
3.1.3.2 Mn2+ enzyme contains 0.3 mol of Mn2+ per mol of enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 30000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 isoenzyme A, B and C 103 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 osteoclastoma cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 387
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-naphthyl phosphate + H2O
-
 Homo sapiens 1-naphthol + phosphate
-
 ?
3.1.3.2 4-methylumbelliferyl phosphate + H2O
-
 Homo sapiens 4-methylumbelliferone + phosphate
-
 ?
3.1.3.2 ADP + H2O
-
 Homo sapiens AMP + phosphate
-
 ?
3.1.3.2 ATP + H2O
-
 Homo sapiens ADP + phosphate
-
 ?
3.1.3.2 CDP + H2O
-
 Homo sapiens CMP + phosphate
-
 ?
3.1.3.2 CTP + H2O
-
 Homo sapiens CDP + phosphate
-
 ?
3.1.3.2 GDP + H2O
-
 Homo sapiens GMP + phosphate
-
 ?
3.1.3.2 GTP + H2O
-
 Homo sapiens GDP + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Homo sapiens p-nitrophenol + phosphate
-
 ?
3.1.3.2 phenolphthalein diphosphate + H2O
-
 Homo sapiens phenolphthalein monophosphate + phosphate
-
 ?
3.1.3.2 phenyl phosphate + H2O
-
 Homo sapiens phenol + phosphate
-
 ?
3.1.3.2 phosphoenolpyruvate + H2O
-
 Homo sapiens pyruvate + phosphate
-
 ?
3.1.3.2 phosphopyruvate + H2O
-
 Homo sapiens pyruvate + phosphate
-
 ?
3.1.3.2 TDP + H2O
-
 Homo sapiens TMP + phosphate
-
 ?
3.1.3.2 TTP + H2O
-
 Homo sapiens TDP + phosphate
-
 ?
3.1.3.2 UDP + H2O
-
 Homo sapiens UDP + phosphate
-
 ?
3.1.3.2 UTP + 2 H2O
-
 Homo sapiens UMP + 2 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 monomer 1 * 30000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.2 2
-
 phosphoenolpyruvate
-
 Homo sapiens
3.1.3.2 53
-
 Alpha-naphthyl phosphate
-
 Homo sapiens
3.1.3.2 58
-
 CDP
-
 Homo sapiens
3.1.3.2 62
-
 Phenyl phosphate
-
 Homo sapiens
3.1.3.2 69
-
 TDP
-
 Homo sapiens
3.1.3.2 73
-
 ADP
-
 Homo sapiens
3.1.3.2 79
-
 UDP
-
 Homo sapiens
3.1.3.2 86
-
 GDP
-
 Homo sapiens
3.1.3.2 93
-
 CTP
-
 Homo sapiens
3.1.3.2 107
-
 TTP
-
 Homo sapiens
3.1.3.2 109
-
 Phenolphthalein diphosphate
-
 Homo sapiens
3.1.3.2 113
-
 GTP
-
 Homo sapiens
3.1.3.2 119
-
 ATP
-
 Homo sapiens
3.1.3.2 119
-
 UTP
-
 Homo sapiens
3.1.3.2 121
-
 4-methylumbelliferyl phosphate
-
 Homo sapiens
3.1.3.2 198
-
 p-nitrophenyl phosphate
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.5 5.7 in sodium acetate buffer for all substrates Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.2 4 7 stable Homo sapiens