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Literature summary extracted from

  • Damuni, Z.; Reed, L.J.
    Purification and properties of the catalytic subunit of the branched-chain alpha-keto acid dehydrogenase phosphatase from bovine kidney mitochondria (1987), J. Biol. Chem., 262, 5129-5132.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.52 acetyl-CoA
-
 Bos taurus
3.1.3.52 ADP inhibition is completely reversed by Mg2+; inhibition is completely reversed by spermine Bos taurus
3.1.3.52 ATP completely reversed by spermine; inhibition is completely reversed by Mg2+ Bos taurus
3.1.3.52 CoA
-
 Bos taurus
3.1.3.52 GDP inhibition is completely reversed by Mg2+; inhibition is completely reversed by spermine Bos taurus
3.1.3.52 GTP inhibition is completely reversed by Mg2+; inhibition is completely reversed by spermine Bos taurus
3.1.3.52 Inhibitor protein BCKDH phosphatase inhibitor protein, inhibits at nanomolar concentrations Bos taurus
3.1.3.52 isobutyryl-CoA
-
 Bos taurus
3.1.3.52 isovaleryl-CoA
-
 Bos taurus
3.1.3.52 additional information little inhibitory effect: nucleoside monophosphates; little inhibitory effect: protein phosphatase inhibitor 1 and inhibitor 2 Bos taurus
3.1.3.52 nucleoside diphosphates
-
 Bos taurus
3.1.3.52 nucleoside triphosphates
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.16 mitochondrion
-
 Bos taurus 5739
-
3.1.3.52 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.52 33000
-
 1 * 33000, catalytic subunit, SDS-PAGE Bos taurus
3.1.3.52 460000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.52 [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate + H2O Bos taurus
-
 [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Bos taurus
-
-
-
3.1.3.52 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.16 catalytic subunit Bos taurus
3.1.3.52 catalytic subunit Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.16 kidney
-
 Bos taurus
-
3.1.3.52 kidney
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.52 2.328
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.1.3.52 -20°C, stable for at least 6 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 phosphoproteins + H2O
-
 Bos taurus proteins + phosphate
-
 ?
3.1.3.16 phosphorylated BCKDH complex + H2O i.e. branched-chain alpha-keto acid dehydrogenase, phosphorylated Bos taurus BCKDH complex + phosphate
-
 ?
3.1.3.52 additional information inactive with phosphorylase a and with 4-nitrophenyl phosphate Bos taurus ?
-
 ?
3.1.3.52 [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate + H2O
-
 Bos taurus [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.52 More 1 * 33000, catalytic subunit, SDS-PAGE Bos taurus