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Literature summary extracted from

  • Friedman, S.
    Trehalose-6-phosphate phosphatase from insects (1966), Methods Enzymol., 8, 372-374.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.12 arsenate 10 mM, 10% inhibition Phormia regina
3.1.3.12 Cu2+ 0.6 mM, 50% inhibition in presence of 5 mM Mg2+ Phormia regina
3.1.3.12 F- 25 mM, 70% inhibition Phormia regina
3.1.3.12 Zn2+ 0.6 mM, 80% inhibition in presence of 5 mM Mg2+ Phormia regina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.12 Co2+ enzyme is inactive in absence of a bivalent metal. Requirement is met most effectively by Mg2+ with Co2+, Fe2+ and Mn2+ serving much less adequately Phormia regina
3.1.3.12 Fe2+ enzyme is inactive in absence of a bivalent metal. Requirement is met most effectively by Mg2+ with Co2+, Fe2+ and Mn2+ serving much less adequately Phormia regina
3.1.3.12 Mg2+ enzyme is inactive in absence of a bivalent metal. Requirement is met most effectively by Mg2+ with Co2+, Fe2+ and Mn2+ serving much less adequately Phormia regina
3.1.3.12 Mn2+ enzyme is inactive in absence of a bivalent metal. Requirement is met most effectively by Mg2+ with Co2+, Fe2+ and Mn2+ serving much less adequately Phormia regina

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.12 Phormia regina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.12
-
 Phormia regina

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.12 2.61
-
-
 Phormia regina

Storage Stability

EC Number Storage Stability Organism
3.1.3.12 -12°C, completely stable for at least 2 months Phormia regina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.12 trehalose-6-phosphate + H2O
-
 Phormia regina trehalose + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.12 46
-
-
 Phormia regina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.12 57
-
 15 min, 15% loss of activity Phormia regina

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.12 0.6
-
 Cu2+ in presence of 5 mM Mg2+ Phormia regina