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Literature summary extracted from

  • Turner, D.H.; Turner, J.F.
    The hydrolysis of glucose monophosphates by a phosphatase preparation from pea seeds (1960), Biochem. J., 74, 486-491.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.10 HgCl2 74% inhibition of D-glucose 1-phosphate hydrolysis by 0.2 mM Pisum sativum
3.1.3.10 iodoacetic acid 69% inhibition of D-glucose 1-phosphate hydrolysis by 10 mM Pisum sativum
3.1.3.10 Na2MoO4 100% inhibition of D-glucose 1-phosphate hydrolysis by 1.0 mM Pisum sativum
3.1.3.10 NaF 75% inhibition of D-glucose 1-phosphate hydrolysis by 2.0 mM, 100% inhibition of D-glucose 1-phosphate hydrolysis by 20 mM Pisum sativum
3.1.3.10 phosphate 36% inhibition of D-glucose 1-phoshate hydrolysis by 0.075 mM, 52% inhibition of D-glucose 1-phosphate hydrolysis by 0.15 mM Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.10 D-glucose 1-phosphate + H2O Pisum sativum
-
 D-glucose + phosphate
-
 ?
3.1.3.10 D-glucose 6-phosphate + H2O Pisum sativum
-
 D-glucose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.10 Pisum sativum
-
 L.var. 99L, var.Alaska
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.10
-
 Pisum sativum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.10 100
-
 purification procedure described Pisum sativum
3.1.3.10 100
-
 with D-glucose 1-phosphate as substrate Pisum sativum
3.1.3.10 637
-
 purification procedure described Pisum sativum
3.1.3.10 637
-
 with D-glucose 6-phosphate as substrate Pisum sativum

Storage Stability

EC Number Storage Stability Organism
3.1.3.10 -15 ºC, water, 2 months, little loss of activity Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.10 3-phosphoglycerate + H2O hydrolysis is more rapid than that of glucose monophosphates Pisum sativum 2,3-dihydroxypropanoic acid + phosphate
-
 ?
3.1.3.10 4-nitrophenyl phosphate + H2O hydrolysis is more rapid than that of glucose monophosphate Pisum sativum 4-nitrophenol + phosphate
-
 ?
3.1.3.10 AMP + H2O
-
 Pisum sativum adenosine + phosphate
-
 ?
3.1.3.10 ATP + H2O hydrolysis more rapid than that of glucose monophosphates Pisum sativum ADP + phosphate
-
 ?
3.1.3.10 D-fructose 1,6-diphosphate + H2O hydrolysis was more rapid than that of glucose monophosphates Pisum sativum D-fructose + phosphate
-
 ?
3.1.3.10 D-fructose 1-phosphate + H2O
-
 Pisum sativum D-fructose + phosphate
-
 ?
3.1.3.10 D-fructose 6-phosphate + H2O
-
 Pisum sativum D-fructose + phosphate
-
 ?
3.1.3.10 D-glucose 1-phosphate + H2O
-
 Pisum sativum D-glucose + phosphate
-
 ?
3.1.3.10 D-glucose 6-phosphate + H2O
-
 Pisum sativum D-glucose + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.10 glucose monophosphatase
-
 Pisum sativum
3.1.3.10 pea-seed acid phosphatase
-
 Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.10 30
-
 temperature used in the assay with D-glucose 1-phosphate in 0.75mM Tris-acetic acid-buffer, pH5.4 Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.10 5.4
-
 with 0.5 mM D-glucose 6-phosphate as substrate Pisum sativum
3.1.3.10 5.5 5.7 with 0,5 mM D-glucose 1-phosphate as substrate Pisum sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.10 4 7.5 about 40% of maximal activity at pH 6.6 with 0.5 mM D-glucose 1-phosphate or with 0.5 mM D-glucose 6-phosphate Pisum sativum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.10 0.1
-
 Na2MoO4
-
 Pisum sativum