BRENDA - Enzyme Database show

The ribonucleases: occurence, structure and properties

Anfinsen, C.B.; White, F.H.; The Enzymes, 2nd Ed. (Boyer, P. D. ; Lardy, H. ; Myrb├Ąck, K. , eds. ) 5, 95-122 (1961)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
4.6.1.18
2 M guanidine, activity remains
Bos taurus
4.6.1.18
8 M urea, activity remains
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.6.1.18
arsenite
-
Bos taurus
4.6.1.18
chloride
-
Bos taurus
4.6.1.18
Selenite
-
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.6.1.18
RNA + H2O
Mus musculus
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
?
4.6.1.18
RNA + H2O
Homo sapiens
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
?
4.6.1.18
RNA + H2O
Rattus norvegicus
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
?
4.6.1.18
RNA + H2O
Sus scrofa
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
?
4.6.1.18
RNA + H2O
Bos taurus
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.6.1.18
Bos taurus
-
-
-
4.6.1.18
Homo sapiens
-
-
-
4.6.1.18
Mus musculus
-
-
-
4.6.1.18
Rattus norvegicus
-
-
-
4.6.1.18
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.6.1.18
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.6.1.18
pancreas
-
Mus musculus
-
4.6.1.18
pancreas
-
Rattus norvegicus
-
4.6.1.18
pancreas
-
Bos taurus
-
4.6.1.18
pancreas
-
Sus scrofa
-
4.6.1.18
pancreas
-
Homo sapiens
-
4.6.1.18
spleen
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.6.1.18
cyclic 2',3'-nucleoside monophosphate + H2O
second step of hydrolysis is irreversible
134523
Mus musculus
3'-phosphomononucleotides
-
-
-
ir
4.6.1.18
cyclic 2',3'-nucleoside monophosphate + H2O
second step of hydrolysis is irreversible
134523
Homo sapiens
3'-phosphomononucleotides
-
-
-
ir
4.6.1.18
cyclic 2',3'-nucleoside monophosphate + H2O
second step of hydrolysis is irreversible
134523
Rattus norvegicus
3'-phosphomononucleotides
-
-
-
ir
4.6.1.18
cyclic 2',3'-nucleoside monophosphate + H2O
second step of hydrolysis is irreversible
134523
Sus scrofa
3'-phosphomononucleotides
-
-
-
ir
4.6.1.18
cyclic 2',3'-nucleoside monophosphate + H2O
second step of hydrolysis is irreversible
134523
Bos taurus
3'-phosphomononucleotides
-
-
-
ir
4.6.1.18
RNA + H2O
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
134523
Mus musculus
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
-
?
4.6.1.18
RNA + H2O
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
134523
Homo sapiens
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
-
?
4.6.1.18
RNA + H2O
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
134523
Rattus norvegicus
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
-
?
4.6.1.18
RNA + H2O
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
134523
Sus scrofa
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
-
?
4.6.1.18
RNA + H2O
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
134523
Bos taurus
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
-
?
4.6.1.18
RNA + H2O
first step of hydrolysis is reversible
134523
Mus musculus
cyclic 2',3'-nucleoside monophosphate
-
-
-
r
4.6.1.18
RNA + H2O
first step of hydrolysis is reversible
134523
Homo sapiens
cyclic 2',3'-nucleoside monophosphate
-
-
-
r
4.6.1.18
RNA + H2O
first step of hydrolysis is reversible
134523
Rattus norvegicus
cyclic 2',3'-nucleoside monophosphate
-
-
-
r
4.6.1.18
RNA + H2O
first step of hydrolysis is reversible
134523
Sus scrofa
cyclic 2',3'-nucleoside monophosphate
-
-
-
r
4.6.1.18
RNA + H2O
first step of hydrolysis is reversible
134523
Bos taurus
cyclic 2',3'-nucleoside monophosphate
-
-
-
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.6.1.18
6.5
-
-
Rattus norvegicus
4.6.1.18
7
7.5
-
Bos taurus
4.6.1.18
7
-
-
Mus musculus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.6.1.18
7
7.8
-
Bos taurus
4.6.1.18
7
7.8
-
Homo sapiens
4.6.1.18
7
7.8
-
Rattus norvegicus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.6.1.18
2
-
activity remains below pH 2
Bos taurus
General Stability (protein specific)
EC Number
General Stability
Organism
4.6.1.18
2 M guanidine, activity remains
Bos taurus
4.6.1.18
8 M urea, activity remains
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.6.1.18
arsenite
-
Bos taurus
4.6.1.18
chloride
-
Bos taurus
4.6.1.18
Selenite
-
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.6.1.18
RNA + H2O
Mus musculus
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
?
4.6.1.18
RNA + H2O
Homo sapiens
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
?
4.6.1.18
RNA + H2O
Rattus norvegicus
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
?
4.6.1.18
RNA + H2O
Sus scrofa
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
?
4.6.1.18
RNA + H2O
Bos taurus
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.6.1.18
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.6.1.18
pancreas
-
Mus musculus
-
4.6.1.18
pancreas
-
Sus scrofa
-
4.6.1.18
pancreas
-
Rattus norvegicus
-
4.6.1.18
pancreas
-
Homo sapiens
-
4.6.1.18
pancreas
-
Bos taurus
-
4.6.1.18
spleen
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.6.1.18
cyclic 2',3'-nucleoside monophosphate + H2O
second step of hydrolysis is irreversible
134523
Mus musculus
3'-phosphomononucleotides
-
-
-
ir
4.6.1.18
cyclic 2',3'-nucleoside monophosphate + H2O
second step of hydrolysis is irreversible
134523
Homo sapiens
3'-phosphomononucleotides
-
-
-
ir
4.6.1.18
cyclic 2',3'-nucleoside monophosphate + H2O
second step of hydrolysis is irreversible
134523
Rattus norvegicus
3'-phosphomononucleotides
-
-
-
ir
4.6.1.18
cyclic 2',3'-nucleoside monophosphate + H2O
second step of hydrolysis is irreversible
134523
Sus scrofa
3'-phosphomononucleotides
-
-
-
ir
4.6.1.18
cyclic 2',3'-nucleoside monophosphate + H2O
second step of hydrolysis is irreversible
134523
Bos taurus
3'-phosphomononucleotides
-
-
-
ir
4.6.1.18
RNA + H2O
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
134523
Mus musculus
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
-
?
4.6.1.18
RNA + H2O
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
134523
Homo sapiens
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
-
?
4.6.1.18
RNA + H2O
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
134523
Rattus norvegicus
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
-
?
4.6.1.18
RNA + H2O
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
134523
Sus scrofa
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
-
?
4.6.1.18
RNA + H2O
endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA
134523
Bos taurus
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
-
-
?
4.6.1.18
RNA + H2O
first step of hydrolysis is reversible
134523
Mus musculus
cyclic 2',3'-nucleoside monophosphate
-
-
-
r
4.6.1.18
RNA + H2O
first step of hydrolysis is reversible
134523
Homo sapiens
cyclic 2',3'-nucleoside monophosphate
-
-
-
r
4.6.1.18
RNA + H2O
first step of hydrolysis is reversible
134523
Rattus norvegicus
cyclic 2',3'-nucleoside monophosphate
-
-
-
r
4.6.1.18
RNA + H2O
first step of hydrolysis is reversible
134523
Sus scrofa
cyclic 2',3'-nucleoside monophosphate
-
-
-
r
4.6.1.18
RNA + H2O
first step of hydrolysis is reversible
134523
Bos taurus
cyclic 2',3'-nucleoside monophosphate
-
-
-
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.6.1.18
6.5
-
-
Rattus norvegicus
4.6.1.18
7
7.5
-
Bos taurus
4.6.1.18
7
-
-
Mus musculus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.6.1.18
7
7.8
-
Bos taurus
4.6.1.18
7
7.8
-
Homo sapiens
4.6.1.18
7
7.8
-
Rattus norvegicus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.6.1.18
2
-
activity remains below pH 2
Bos taurus