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Literature summary extracted from

  • Andrews, A.J.; Brown, J.W.
    The partial purification of Methanococcus jannaschii RNase P holoenzyme (1999), Nucleic Acids Symp. Ser., 41, 60-62.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.26.5 32
-
pre-tRNAAsp
-
Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.5 Mg2+ maximally active at 2.5-30 mM MgCl2 Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.5 400000
-
greater than, gel filtration Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.5 Methanocaldococcus jannaschii
-
-
-
3.1.26.5 Methanothermobacter thermautotrophicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.26.5 ribonucleoprotein RNase P RNA is catalytically active in the absence of any protein in the appropriate ionic conditions Methanothermobacter thermautotrophicus
3.1.26.5 ribonucleoprotein the RNase P RNA lacks essential secondary structures required for the recognition of pre-tRNA and as a result is not catalytically active in vitro Methanocaldococcus jannaschii

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.5
-
Methanothermobacter thermautotrophicus
3.1.26.5 partial. The RNase P RNA lacks essential secondary structures required for the recognition of pre-tRNA and as a result is not catalytically active in vitro Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.5 pre-tRNA precursor + H2O pre-tRNAAsp Methanothermobacter thermautotrophicus tRNA + 5'-oligoribonucleotide
-
?
3.1.26.5 pre-tRNA precursor + H2O pre-tRNAAsp Methanocaldococcus jannaschii tRNA + 5'-oligoribonucleotide
-
?
3.1.26.5 pre-tRNAAsp + H2O
-
Methanocaldococcus jannaschii mature tRNAAsp + 5'-oligonucleotide
-
?