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Literature summary extracted from

  • Cathala, G.; Rech, J.; Huet, J.; Jeanteur, P.
    Isolation and characterization of two types of ribonucleases H in Krebs II ascites cells (1979), J. Biol. Chem., 254, 7353-7361.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.26.4 2-mercaptoethanol enzyme form H2 is inactive in absence of mercaptoethanol. No effect on enzyme form H1 Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.4 Dextran
-
 Mus musculus
3.1.26.4 EDTA
-
 Mus musculus
3.1.26.4 Ethidium bromide
-
 Mus musculus
3.1.26.4 KCl activity of enzyme form H1 decreases rapidly above 50 mM and becomes nearly abolished at 150 mM Mus musculus
3.1.26.4 NaCl activity of enzyme form H1 decreases rapidly above 50 mM and becomes nearly abolished at 150 mM Mus musculus
3.1.26.4 NEM enzyme form H2. No effect on enzyme form H1 Mus musculus
3.1.26.4 NH4Cl activity of enzyme form H1 decreases rapidly above 50 mM and becomes nearly abolished at 150 mM Mus musculus
3.1.26.4 Nucleic acids enzyme form H1 is more susceptible to inhibition than enzyme form H2 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.26.4 additional information
-
 additional information
-
 Mus musculus
3.1.26.4 0.0005
-
 poly(rAdT) lower than 0.0005 mM Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.4 KCl enzyme form H2 is mostly inactive at low salt and requires 100-200 mM concentration for maximal activity. KCl is more efficient than NaCl Mus musculus
3.1.26.4 Mg2+ enzyme form H2: requirement for divalent metal ion can be satisfied only by Mg2+. Enzyme form H1: requirement for a divalent metal ion can be satisfied by Mg2+ or with a stronger preference with Mn2+ Mus musculus
3.1.26.4 Mn2+ enzyme form H1: requirement for a divalent metal ion can be satisfied by Mg2+ or with a stronger preference with Mn2+ Mus musculus
3.1.26.4 NaCl enzyme form H2 is mostly inactive at low salt and requires 100-200 mM concentration for maximal activity. KCl or NH4Cl is more efficient than NaCl Mus musculus
3.1.26.4 NH4Cl enzyme form H2 is mostly inactive at low salt and requires 100-200 mM concentration for maximal activity. NH4Cl is more efficient than NaCl Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.4 33000
-
 x * 33000, enzyme form H2, SDS-PAGE Mus musculus
3.1.26.4 36000 40000 enzyme form H2, gel filtration Mus musculus
3.1.26.4 105000 110000 enzyme form H1, gel filtration Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.4 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.4 partial, two types of enzyme: H1 and H2 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.26.4 Krebs ascites cell
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.4 poly(rAdT) + H2O
-
 Mus musculus ?
-
 ?
3.1.26.4 RNA-DNA hybrid + H2O poly(rAdT) Mus musculus ribonucleotide 5'-phosphomonoester
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.26.4 ? x * 33000, enzyme form H2, SDS-PAGE Mus musculus