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Literature summary extracted from

  • Banks, G.R.
    A ribonuclease H from Ustilago maydis. Properties, mode of action and substrate specificity of the enzyme (1974), Eur. J. Biochem., 47, 499-507.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.26.4 (NH4)2SO4 4fold stimulation at an optimal concentration of 18 mM Ustilago maydis
3.1.26.4 2-mercaptoethanol stimulates Ustilago maydis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.4 (NH4)2SO4 above 60 mM Ustilago maydis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.4 Mg2+
-
Ustilago maydis
3.1.26.4 Mn2+ Mn2+ or Mg2+ required Ustilago maydis
3.1.26.4 Mn2+ optimal concentration: 1.2 mM Ustilago maydis
3.1.26.4 NaCl stimulates Ustilago maydis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.4 100000 130000 gel filtration Ustilago maydis

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.4 Ustilago maydis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.4
-
Ustilago maydis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.26.4 additional information
-
-
Ustilago maydis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.4 RNA-DNA hybrid + H2O can degrade about 90% of the RNA strand of and RNA-DNA hybrid Ustilago maydis ribonucleotide 5'-phosphomonoester
-
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Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.4 32
-
-
Ustilago maydis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.4 7.5 8 Tris buffer or potassium phosphate buffer Ustilago maydis