EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.26.4 | sulfhydryl reagent | required for maximal activity of the Mn2+-dependent enzyme and the Mg2+-dependent enzyme | Gallus gallus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.26.4 | 1,10-phenanthroline | inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme | Gallus gallus | |
3.1.26.4 | diphosphate | inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme | Gallus gallus | |
3.1.26.4 | NaF | inhibition of Mg2+-dependent enzyme | Gallus gallus | |
3.1.26.4 | NEM | 2 mM, 80% inhibition of Mn2+-dependent enzyme, complete inhibition of Mg2+-dependent enzyme | Gallus gallus | |
3.1.26.4 | PCMB | 2 mM, complete inhibition of Mn2+-dependent enzyme and Mg2+-dependent enzyme | Gallus gallus | |
3.1.26.4 | spermidine | inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme | Gallus gallus | |
3.1.26.4 | spermine | inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme | Gallus gallus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.26.4 | Mg2+ | Mg2+-dependent enzyme requires 15-20 mM Mg2+ for maximal activity | Gallus gallus | |
3.1.26.4 | Mg2+ | optimal activity with 10-15 mM | Gallus gallus | |
3.1.26.4 | Mn2+ | 0.4 mM Mn2+ required for optimal activity, some activity with Mg2+ | Gallus gallus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.26.4 | 40000 | - |
Mg2+-dependent enzyme, gel filtration | Gallus gallus |
3.1.26.4 | 120000 | - |
Mn2+-dependent enzyme, gel filtration | Gallus gallus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.26.4 | Gallus gallus | - |
one Mn2+-dependent RNase H and one Mg2+-dependent RNase H | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.26.4 | one Mn2+-dependent RNase H and one Mg2+-dependent RNase H | Gallus gallus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.26.4 | embryo | - |
Gallus gallus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.26.4 | additional information | - |
- |
Gallus gallus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.26.4 | RNA-DNA hybrid + H2O | - |
Gallus gallus | ribonucleotide 5'-phosphomonoester | a mixture of oligoribonucleotides with 5'-phosphate and 3'-hydroxyl terminus | ? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.26.4 | 8.5 | - |
Mn2+-dependent enzyme | Gallus gallus |
3.1.26.4 | 9.5 | - |
Mg2+-dependent enzyme | Gallus gallus |