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Literature summary extracted from

  • Sawai, Y.; Yanokura, M.; Tsukada, K.
    Multiple forms of ribonuclease H from rat liver cytosol (1979), J. Biochem., 86, 757-764.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.26.4 (NH4)2SO4 100 mM, isoenzyme I and II are activated by 50%. Isoenzyme II shows optimal activity at 50 mM, 80% inhibition at 200 mM Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.4 (NH4)2SO4 isoenzyme II, 80% inhibition at 200 mM Rattus norvegicus
3.1.26.4 PCMB in absence of 2-mercaptoethanol, isoenzyme I and III are completely inhibited by 0.2 mM PCMB, Activity of isoenzyme II is inhibited 20% Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.26.4 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.4 Mg2+ isoenzyme I and II both require 10-15 mM Mg2+ for maximal activity. Isoenzyme II is maximally activated by Mg2+, some activity with Mn2+ Rattus norvegicus
3.1.26.4 Mn2+ isoenzyme II is maximally active at 0.4 mM, some activity with Mg2+ Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.4 isoenzyme I, II and III Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.26.4 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.26.4 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.4 RNA-DNA hybrid + H2O poly(rAdT) Rattus norvegicus ribonucleotide 5'-phosphomonoester oligonucleotides with 3'-hydroxyl and 5'-phosphate termini with the structure (pA)3-9 are formed from poly(A)*poly(dT) ?