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Literature summary extracted from
- Studies on two nucleases and a ribonuclease from Physarum polycephalum. Purification and mode of action (1969), J. Biochem., 65, 701-708.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.26.1 | analysis | - |
Physarum polycephalum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.1 | EDTA | - |
Physarum polycephalum |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.1 | Zn2+ | strongly activating | Physarum polycephalum | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.1 | RNA + H2O | Physarum polycephalum | - |
nucleoside 5'-monophosphates | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.26.1 | Physarum polycephalum | - |
slime mold | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.26.1 | - |
Physarum polycephalum |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.1.26.1 | Endonucleolytic cleavage to a 5'-phosphomonoester | purine preference with regard to 3'-terminal nucleotides | Physarum polycephalum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.1 | DNA + H2O | - |
Physarum polycephalum | nucleoside 5'-monophosphates | - |
? | |
| 3.1.26.1 | RNA + H2O | - |
Physarum polycephalum | nucleoside 5'-monophosphates | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.1 | 4 | - |
pH 4.5 for 12 hours : 55% of activity remains, pH 6.5 for 12 hours : 50% of activity remains, pH 8.5 for 12 hours : 40% of activity remains | Physarum polycephalum |
| 3.1.26.1 | 37 | - |
pH 4.5 for 1 h : 60% of activity remains, pH 4.5 for 12 hours : 20% of activity remains | Physarum polycephalum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.1 | 4 | - |
- |
Physarum polycephalum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.1 | 6.5 | - |
inactivation above this value | Physarum polycephalum |
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Release 2023.1 (January 2023)
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