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Literature summary extracted from

  • Takahagi, M.; Iwasaki, H.; Shinagawa, H.
    Structural requirements of substrate DNA for binding to and cleavage by RuvC, a holliday junction resolvase (1994), J. Biol. Chem., 269, 15132-15139.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.10 KCl reduces activity 10fold at a concentration of 200 mM Escherichia coli
3.1.21.10 potassium glutamate reduces activity 10fold at a concentration of 200 mM Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.10 Ca2+ poor substitute for Mg2+ Escherichia coli
3.1.21.10 Co2+ at 25 mM moderately effective for activity Escherichia coli
3.1.21.10 Mg2+ optimum: 8-25 mM, binding per se is independent of Mg2+, cleavage mechanism depends on Mg2+ Escherichia coli
3.1.21.10 Mg2+ required for activation Escherichia coli
3.1.21.10 Mn2+ can substitute for Mg2+, but is required in higher amounts Escherichia coli
3.1.21.10 Zn2+ poor substitute for Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.10 DNA + H2O Escherichia coli holliday structure hydrolyzed DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.10 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.10 DNA + H2O nicks the ssDNA strands across the junction at symmetrical positions within the homologous arms Escherichia coli hydrolyzed DNA
-
 ?
3.1.21.10 DNA + H2O sequence specific: 5'- ATT - cleavage site - G-3', substrate: homologous core of 12 bp between the arms is required for cleavage but not for binding of the enzyme to the substrate, cleaves also Y-junctions with homologous core Escherichia coli hydrolyzed DNA
-
 ?
3.1.21.10 DNA + H2O holliday structure Escherichia coli hydrolyzed DNA
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.10 7 9
-
 Escherichia coli