Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dulaney, J.T.; Touster, O.
    Isolation of deoxyribonuclease II of rat liver lysosomes (1972), J. Biol. Chem., 247, 1424-1432.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.22.1 addition of bovine serum albumin stabilizes enzyme activity at 37°C Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.22.1 iodoacetate 85% inhibition at 5 mM Rattus sp.
3.1.22.1 Mn(CH3COO)2 32% inhibition at 20 mM Rattus sp.
3.1.22.1 SO42- 68% inhibition at 20 mM Rattus sp.
3.1.22.1 Zn(CH3COO)2 67% inbhibition at 20 mM Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.22.1 lysosome
-
 Rattus sp. 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.22.1 36000
-
 gel filtration Rattus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.22.1 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.22.1 to homogeneity by sucrose centrifugation steps and chromatography steps Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.22.1 liver
-
 Rattus sp.
-

Storage Stability

EC Number Storage Stability Organism
3.1.22.1 8°C, 0.4 M NaCl, 0.05 M acetate, pH 5.5, one third of activity after 1 year Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.22.1 DNA + H2O calf thymus DNA, micrococcus luteus DNA and poly[d(A-T)d(A-T)] Rattus sp. 3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides formation of dinucleotides to 8 nucleotide chain length ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.22.1 4.1
-
-
 Rattus sp.