Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Baker, K.P.; Baron, W.F.; Henzel, W.J.; Spencer, S.A.
    Molecular cloning and characterization of human and murine DNase II (1998), Gene, 215, 281-289.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.22.1 expression in human embryonic kidney 293 cells Mus musculus
3.1.22.1 expression in human embryonic kidney 293 cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.22.1 extracellular 20-30% secreted Mus musculus
-
-
3.1.22.1 extracellular 20-30% secreted Homo sapiens
-
-
3.1.22.1 extracellular 20-30% secreted Sus scrofa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.22.1 10000
-
 1 * 35000 + 1 * 10000, protein sequence analysis Sus scrofa
3.1.22.1 35000
-
 1 * 35000 + 1 * 10000, protein sequence analysis Sus scrofa
3.1.22.1 38030
-
 calculation from cDNA sequence Homo sapiens
3.1.22.1 42000 44000 SDS-PAGE, mature protein Homo sapiens
3.1.22.1 45000
-
-
 Homo sapiens
3.1.22.1 45000
-
 protein sequence analysis of a 35000 and 10000 Da protein band Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.22.1 DNA + H2O Mus musculus directed into the secretory pathway oligonucleotides + ?
-
 ?
3.1.22.1 DNA + H2O Homo sapiens directed into the secretory pathway oligonucleotides + ?
-
 ?
3.1.22.1 DNA + H2O Sus scrofa directed into the secretory pathway oligonucleotides + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.22.1 Homo sapiens
-
-
-
3.1.22.1 Mus musculus
-
-
-
3.1.22.1 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.22.1 glycoprotein
-
 Homo sapiens
3.1.22.1 glycoprotein
-
 Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.22.1 commercially available DNase II further purified by chromatography steps to homogeneity Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.22.1 cell culture
-
 Mus musculus
-
3.1.22.1 cell culture
-
 Homo sapiens
-
3.1.22.1 liver
-
 Mus musculus
-
3.1.22.1 liver
-
 Homo sapiens
-
3.1.22.1 liver
-
 Sus scrofa
-
3.1.22.1 placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.22.1 DNA + H2O
-
 Sus scrofa oligonucleotides + ?
-
 ?
3.1.22.1 DNA + H2O salmon sperm DNA Mus musculus oligonucleotides + ?
-
 ?
3.1.22.1 DNA + H2O salmon sperm DNA Homo sapiens oligonucleotides + ?
-
 ?
3.1.22.1 DNA + H2O directed into the secretory pathway Mus musculus oligonucleotides + ?
-
 ?
3.1.22.1 DNA + H2O directed into the secretory pathway Homo sapiens oligonucleotides + ?
-
 ?
3.1.22.1 DNA + H2O directed into the secretory pathway Sus scrofa oligonucleotides + ?
-
 ?
3.1.22.1 supercoiled plasmid DNA + H2O plasmid pBR322 Mus musculus relaxed plasmid DNA + ? primary reaction product, "nicking" mechanism ?
3.1.22.1 supercoiled plasmid DNA + H2O plasmid pBR322 Homo sapiens relaxed plasmid DNA + ? primary reaction product, "nicking" mechanism ?

Subunits

EC Number Subunits Comment Organism
3.1.22.1 dimer 1 * 35000 + 1 * 10000, protein sequence analysis Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.22.1 4.6
-
-
 Mus musculus
3.1.22.1 4.6
-
-
 Homo sapiens