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Literature summary extracted from

  • Janscak, P.; Abadjieva, A.; Firman, K.
    The type I restriction endonuclease R.EcoR124I: Over-production and biochemical properties (1996), J. Mol. Biol., 257, 977-991.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.21.3 S-adenosyl-L-methionine stimulates, not required Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.3 DNA cleavage of DNA is inhibited by an increased degree of negative supercoiling Escherichia coli
3.1.21.3 S-adenosyl homocysteine competitive Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.3 Mg2+ required Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.3 290000 315000 pentameric enzyme form R2M2S1, gel filtration Escherichia coli
3.1.21.3 312000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.3 Escherichia coli
-
 enzyme R.EcoR124I
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.3
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.3 duplex DNA + ATP the enzyme is both a restriction endonuclease and a modification methylase. Hemi-methylated DNA is the preferred substrate for methylation Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate
-
 ?
3.1.21.3 duplex DNA + ATP supercoiled with one or two SR124I recognition sites is cleaved by the same mechanism. Nicked-circle DNA is an intermediate of the cleavage reaction Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.21.3 More the stoichiometry of R.EcoR124 appears to be R1M2S1, the stoichiometry of R.EcoKI is R2M2S1. S is the HsdS-subunit which is responsible for DNA recognition, R is HsdR-subunit which is required for restriction and M is an independent methyltransferase, Mtase Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.3 6
-
 EcoB, methylase activity Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
3.1.21.3 ATP required Escherichia coli