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Literature summary extracted from

  • Sadowski, P.D.; Hurwitz, J.
    Enzymatic breakage of deoxyribonucleic acid. II. Purification and properties of endonuclease IV from T4 phage-infected Escherichia coli (1969), J. Biol. Chem., 244, 6192-6198.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.21.2 2-mercaptoethanol slight activation Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.2 (NH4)2SO4 0.05 M Escherichia coli
3.1.21.2 KCl 0.05 M Escherichia coli
3.1.21.2 NaCl 0.05 M Escherichia coli
3.1.21.2 NH4Cl 0.05 M Escherichia coli
3.1.21.2 p-hydroxymercuribenzoate
-
 Escherichia coli
3.1.21.2 potassium phosphate buffer pH 8.3 Escherichia coli
3.1.21.9 (NH4)2SO4 0.05 M, 70% inhibition Tequatrovirus T4
3.1.21.9 K3PO4 0.05 M, 80% inhibition Tequatrovirus T4
3.1.21.9 KCl 0.05 M, 80% inhibition Tequatrovirus T4
3.1.21.9 NaCl 0.05 M, 85% inhibition Tequatrovirus T4
3.1.21.9 NH4Cl 0.05 M, 47% inhibition Tequatrovirus T4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.2 CaCl2 8% of the activity found compared to optimal Mg2+ concentration Escherichia coli
3.1.21.2 CoCl2 can substitute for MgCl2, optimal concentration: 0.01 M, activity 27% higher than at optimal MgCl2 concentration Escherichia coli
3.1.21.2 MgCl2 absolute requirement, no activity in absence, optimal concentration: 0.01 M Escherichia coli
3.1.21.2 Mn2+ 0.01 M can replace Mg2+ Escherichia coli
3.1.21.9 Co2+ CoCl2 can substitute for MgCl2. The activity at the optimal Co2+ concentration (0.001 M) is 27% higher than that found at the optimal Mg2+ concentration Tequatrovirus T4
3.1.21.9 Mg2+ optimal concentration is 0.01 M. The activity at 0.001 M and 0.05 M MgCl2 is 50% of that at 0.01 M Tequatrovirus T4
3.1.21.9 Mn2+ Mn2+ (0.001 M) quantitatively can replace Mg2+ in the reaction Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.2 single-stranded DNA + H2O Escherichia coli breakdown of host cell DNA occuring after T4 phage infection 5'-phosphooligonucleotides
-
 ir
3.1.21.2 single-stranded DNA + H2O Escherichia coli B / ATCC 11303 breakdown of host cell DNA occuring after T4 phage infection 5'-phosphooligonucleotides
-
 ir
3.1.21.9 DNA + H2O Tequatrovirus T4 the phage T4 enzyme is involved in degradation of host DNA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.2 Escherichia coli
-
-
-
3.1.21.2 Escherichia coli
-
 phage T4 infected
-
3.1.21.2 Escherichia coli B / ATCC 11303
-
-
-
3.1.21.9 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.2 phage T4 infected Escherichia coli
3.1.21.9 purified about 150fold from T4 phage-infected Escherichia coli B Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.2 single-stranded DNA + H2O preference for single-stranded DNA Escherichia coli 5'-phosphooligonucleotides about 150 nucleotides long ir
3.1.21.2 single-stranded DNA + H2O denatured lambda-DNA, breaks exlusively adjacent to cytosine residues Escherichia coli 5'-phosphooligonucleotides about 150 nucleotides long ir
3.1.21.2 single-stranded DNA + H2O no activity on denatured T4 DNA Escherichia coli 5'-phosphooligonucleotides about 150 nucleotides long ir
3.1.21.2 single-stranded DNA + H2O breakdown of host cell DNA occuring after T4 phage infection Escherichia coli 5'-phosphooligonucleotides
-
 ir
3.1.21.2 single-stranded DNA + H2O preference for single-stranded DNA Escherichia coli B / ATCC 11303 5'-phosphooligonucleotides about 150 nucleotides long ir
3.1.21.2 single-stranded DNA + H2O denatured lambda-DNA, breaks exlusively adjacent to cytosine residues Escherichia coli B / ATCC 11303 5'-phosphooligonucleotides about 150 nucleotides long ir
3.1.21.2 single-stranded DNA + H2O no activity on denatured T4 DNA Escherichia coli B / ATCC 11303 5'-phosphooligonucleotides about 150 nucleotides long ir
3.1.21.2 single-stranded DNA + H2O breakdown of host cell DNA occuring after T4 phage infection Escherichia coli B / ATCC 11303 5'-phosphooligonucleotides
-
 ir
3.1.21.9 DNA + H2O the phage T4 enzyme is involved in degradation of host DNA Tequatrovirus T4 ?
-
 ?
3.1.21.9 DNA + H2O the phage T4 enzyme is involved in degradation of host DNA. It introduces breaks preferentially into single stranded DNA. It produces oligonucleotides as small as 150 nucleotides long from fd DNA. These oligonucleotides contain 3'-hydroxyl and 5'-phosphate termini. T4 endonuclease IV produces breaks exclusively adjacent to cytosine residues in both fd and denatured lambda DNA.The enzyme does not cleave double-stranded DNA or native T4 DNA, which contains 5-hydroxymethylcytosine instead of cytosine Tequatrovirus T4 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.9 denB
-
 Tequatrovirus T4
3.1.21.9 EndoIV ambiguous Tequatrovirus T4
3.1.21.9 T4 endonuclease IV
-
 Tequatrovirus T4

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.21.2 38
-
 assay at Escherichia coli
3.1.21.9 38
-
 assay at Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.2 8.4 9.2
-
 Escherichia coli
3.1.21.9 8
-
 assay at Tequatrovirus T4
3.1.21.9 8.4 9.2 measured in 0.05 M Tris or glycine buffer Tequatrovirus T4

General Information

EC Number General Information Comment Organism
3.1.21.9 physiological function the phage T4 enzyme is involved in degradation of host DNA Tequatrovirus T4