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Literature summary extracted from

  • Lu, B.C.; Wong, W.; Fanning, L.; Sakaguchi, K.
    Purification and characterization of an endonuclease from fruiting caps of basidiomycete Coprinuns cinereus (1988), Eur. J. Biochem., 174, 725-732.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.21.1 sensitives to repeated freezing and thawing Coprinopsis cinerea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.1 EGTA activity inhibited Coprinopsis cinerea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.1 Ca2+ in presence of Ca2+ less sensitive to buffer strength Coprinopsis cinerea
3.1.21.1 Mg2+ absolute requirement for activity, highest activity if both Mg2+ and Ca2+ are present Coprinopsis cinerea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.1 12000
-
 SDS-PAGE Coprinopsis cinerea
3.1.21.1 14000
-
 SDS-PAGE Coprinopsis cinerea
3.1.21.1 22000
-
 gel filtration Coprinopsis cinerea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.1 double-stranded DNA + H2O Coprinopsis cinerea preference for single-stranded and supercoiled DNA 5'-phosphooligonucleotides + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Coprinopsis cinerea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.1 basidiocarp
-
 Coprinopsis cinerea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.21.1 additional information
-
 21 units/min/ng DNA digested Coprinopsis cinerea

Storage Stability

EC Number Storage Stability Organism
3.1.21.1 -70°C, 10 mM Tris/HCl, pH 7.4, 50% v/v glycerol, 2 years Coprinopsis cinerea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 double-stranded DNA + H2O preference for single-stranded and supercoiled DNA Coprinopsis cinerea 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 relaxed circular plasmid DNA + H2O
-
 Coprinopsis cinerea 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 single-stranded circular DNA + H2O preference for single-stranded regions Coprinopsis cinerea 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 supercoiled plasmid DNA + H2O
-
 Coprinopsis cinerea linear DNA + ? formation of a relaxed circle that subsequently converts to linear DNA if it is nicked on the other strand at the same position ?

Subunits

EC Number Subunits Comment Organism
3.1.21.1 dimer 1 * 12000 and 1 * 14000, gel filtration, SDS-PAGE Coprinopsis cinerea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.21.1 37
-
-
 Coprinopsis cinerea

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.21.1 37 60 activity remains unchanged Coprinopsis cinerea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.21.1 60
-
 stable for 20 min Coprinopsis cinerea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.1 7
-
-
 Coprinopsis cinerea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.21.1 5 8 64% activity below pH 5.0, 80% activity above pH 8.0 Coprinopsis cinerea