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Literature summary extracted from
- Purification and characterization of tilapia (Oreochromis mossambicus) deoxyribonuclease I. Primary structure and cDNA sequence (1997), Eur. J. Biochem., 249, 786-791.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.21.1 | deducing amino acid composition from cDNA | Oreochromis mossambicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.21.1 | iodoacetate | 50% inhibition at 0.1 M in presence of 4 mM CuCl2 after 15 min | Oreochromis mossambicus |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.21.1 | Mg2+ | highest activity at 10 mM, doubled activity upon addition of 1 mM Ca2+ | Oreochromis mossambicus | |
| 3.1.21.1 | Mn2+ | highest activity at 1 mM, addition of Ca2+ doesn't change activity | Oreochromis mossambicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.1 | 30000 | - |
gel filtration, SDS-PAGE, thin-layer IEF, MS analysis | Oreochromis mossambicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.1 | double-stranded DNA + H2O | Oreochromis mossambicus | - |
5'-phosphooligonucleotides + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.21.1 | Oreochromis mossambicus | - |
tilapia fish | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.21.1 | glycoprotein | carbohydrate-chain at Asn 18 | Oreochromis mossambicus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.21.1 | to homogeneity by 4step chromatography | Oreochromis mossambicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.21.1 | hepatopancreas | - |
Oreochromis mossambicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.1 | additional information | - |
units defined as absorbance at 260 nm/ml assay solution | Oreochromis mossambicus |
| 3.1.21.1 | additional information | - |
670 units/mg protein | Oreochromis mossambicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.1 | double-stranded DNA + H2O | - |
Oreochromis mossambicus | 5'-phosphooligonucleotides + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.21.1 | monomer | 1 * 30000, SDS-PAGE, gel filtration | Oreochromis mossambicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.1 | 55 | - |
inactivation after 10 min incubation | Oreochromis mossambicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.1 | 8.5 | - |
in the presence of Mg2+ | Oreochromis mossambicus |
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