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Literature summary extracted from

  • Inoue, Y.; Kimura, A.
    Purification and characterization of glyoxalase II from Hansenula mrakii (1992), J. Ferment. Bioeng., 73, 271-276.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.6 Cu2+
-
 Cyberlindnera mrakii
3.1.2.6 Hg2+ HgCl2 Cyberlindnera mrakii
3.1.2.6 iodoacetate
-
 Cyberlindnera mrakii
3.1.2.6 methylglyoxal-glutathione hemimercaptal
-
 Cyberlindnera mrakii
3.1.2.6 NEM
-
 Cyberlindnera mrakii
3.1.2.6 PCMB
-
 Cyberlindnera mrakii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.6 0.086
-
 S-Lactoylglutathione
-
 Cyberlindnera mrakii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.6 22000
-
 gel filtration Cyberlindnera mrakii
3.1.2.6 24000
-
-
 Cyberlindnera mrakii

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.6 Cyberlindnera mrakii
-
 IFO 0895
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.6
-
 Cyberlindnera mrakii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.6 31.9
-
-
 Cyberlindnera mrakii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.6 lactoylglutathione + H2O
-
 Cyberlindnera mrakii D-lactate + glutathione
-
 ?
3.1.2.6 S-acetylglutathione + H2O
-
 Cyberlindnera mrakii acetate + glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.6 monomer 1 * 24000, SDS-PAGE Cyberlindnera mrakii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.6 45 50
-
 Cyberlindnera mrakii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.2.6 25 55 25°C: about 60% of maximal activity, 55°C: about 70% of maximal activity Cyberlindnera mrakii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.2.6 50
-
 15 min, 15% loss of activity Cyberlindnera mrakii
3.1.2.6 55
-
 15 min, 90% loss of activity Cyberlindnera mrakii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.6 8
-
-
 Cyberlindnera mrakii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.2.6 6 8 stable Cyberlindnera mrakii