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Literature summary extracted from

  • Uotila, L.
    Purification and characterization of S-2-hydroxyacylglutathione hydrolase (glyoxalase II) from human liver (1973), Biochemistry, 12, 3944-3951.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.2.6 50% loss of activity on freezing and thawing Homo sapiens
3.1.2.6 bovine serum albumin and glycerol, 30% v/v remove the sensitivity to freezing Homo sapiens
3.1.2.6 dialysis, 50% loss of activity after 8 h Homo sapiens
3.1.2.6 EDTA, 1 mM, labilizes the enzyme in long-term storage Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.6 2,4,6-Trinitrobenzenesulfonate
-
 Homo sapiens
3.1.2.6 AsO2-
-
 Homo sapiens
3.1.2.6 CdCl2
-
 Homo sapiens
3.1.2.6 copper acetate above 15 mM Homo sapiens
3.1.2.6 diphosphate 25 mM, 50% inhibition Homo sapiens
3.1.2.6 glutathione weak competitive Homo sapiens
3.1.2.6 Hg2+ HgCl2, most potent inhibitor Homo sapiens
3.1.2.6 KCl above 15 mM Homo sapiens
3.1.2.6 NaCl above 15 mM Homo sapiens
3.1.2.6 NH4Cl above 15 mM Homo sapiens
3.1.2.6 oxalate 20 mM, 38% inhibition Homo sapiens
3.1.2.6 phosphate 67 mM, 35% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.6 0.0164
-
 S-mandeloylglutathione
-
 Homo sapiens
3.1.2.6 0.07
-
 S-glycolylglutathione
-
 Homo sapiens
3.1.2.6 0.109
-
 S-glyceroylglutathione
-
 Homo sapiens
3.1.2.6 0.153
-
 S-formylglutathione
-
 Homo sapiens
3.1.2.6 0.19
-
 S-Lactoylglutathione
-
 Homo sapiens
3.1.2.6 0.2
-
 S-succinylglutathione
-
 Homo sapiens
3.1.2.6 0.213
-
 S-propionylglutathione
-
 Homo sapiens
3.1.2.6 0.266
-
 S-acetylglutathione
-
 Homo sapiens
3.1.2.6 0.296
-
 S-Acetoacetylglutathione
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.6 22900
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.6 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.6
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.6 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.6 822
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.6 lactoylglutathione + H2O
-
 Homo sapiens D-lactate + glutathione
-
 ?
3.1.2.6 S-acetoacetylglutathione + H2O 55.6% of the activity with S-lactoylglutathione Homo sapiens acetoacetate + glutathione
-
 ?
3.1.2.6 S-acetylglutathione + H2O 8.6% of the activity with S-lactoylglutathione Homo sapiens acetate + glutathione
-
 ?
3.1.2.6 S-formylglutathione + H2O 37.7% of the activity with S-lactoylglutathione Homo sapiens formate + glutathione
-
 ?
3.1.2.6 S-glyceroylglutathione + H2O
-
 Homo sapiens glycerate + glutathione
-
 ?
3.1.2.6 S-glycerylglutathione + H2O 61.6% of the activity with S-lactoylglutathione Homo sapiens glycerate + glutathione
-
 ?
3.1.2.6 S-glycoloylglutathione + H2O 38.6% of the activity with S-lactoylglutathione Homo sapiens glycolate + glutathione
-
 ?
3.1.2.6 S-lactoylglutathione + H2O
-
 Homo sapiens D-lactate + glutathione
-
 ?
3.1.2.6 S-mandeloylglutathione + H2O 4.8% of the activity with S-lactoylglutathione Homo sapiens mandelate + glutathione
-
 ?
3.1.2.6 S-propionylglutathione + H2O 13.5% of the activity with S-lactoylglutathione Homo sapiens propionate + glutathione
-
 ?
3.1.2.6 S-succinylglutathione + H2O 29.2% of the activity with S-lactoylglutathione Homo sapiens succinate + glutathione
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.2.6 35
-
 pH 7, 5 min, stable up to Homo sapiens
3.1.2.6 45
-
 pH 7, 5 min, 50% loss of activity Homo sapiens
3.1.2.6 60
-
 pH 7, 5 min, complete inactivation Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.6 6.8 7.5
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.2.6 25
-
 diphosphate
-
 Homo sapiens