BRENDA - Enzyme Database show

Spleen acid exonuclease

Bernardi, A.; Bernardi, G.; The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ) 4, 329-336 (1971)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.16.1
EDTA
with RNA core as substrate
Bos taurus
3.1.16.1
sulfhydryl reagents
with RNA core as substrate
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.16.1
acetylation
complete inhibition by acetylation of the 2'-OH of tRNA
Bos taurus
3.1.16.1
arsenite
weak inhibitor with RNA core as substrate
Bos taurus
3.1.16.1
Cu2+
-
Bos taurus
3.1.16.1
Deoxyribonucleoside 3'-phosphates
-
Bos taurus
3.1.16.1
F-
weak inhibitor with RNA core as substrate
Bos taurus
3.1.16.1
glycosylation
no activity with glycosylated oligonucleotides obtained from T4 phage DNA
Bos taurus
3.1.16.1
Hg2+
with RNA core as substrate
Bos taurus
3.1.16.1
Mg2+
with RNA core as substrate
Bos taurus
3.1.16.1
Mn2+
with RNA core as substrate
Bos taurus
3.1.16.1
Zn2+
with RNA core as substrate
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.16.1
Bos taurus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.16.1
spleen
-
Bos taurus
-
Storage Stability
EC Number
Storage Stability
Organism
3.1.16.1
-15C, in 50% glycerol
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.16.1
4-nitrophenyl phosphate
-
134135
Bos taurus
4-nitrophenol + phosphate
-
134135
Bos taurus
?
3.1.16.1
bis-(4-nitrophenyl)phosphate + H2O
-
134135
Bos taurus
4-nitrophenyl phosphate + 4-nitrophenol
-
134135
Bos taurus
?
3.1.16.1
DNA + H2O
denatured only, no native or double stranded
134135
Bos taurus
deoxyribonucleoside 3'-phosphates
-
134135
Bos taurus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.16.1
5.8
-
substrate bis-(p-nitrophenyl) phosphate in 0.25 M succinate buffer
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.16.1
EDTA
with RNA core as substrate
Bos taurus
3.1.16.1
sulfhydryl reagents
with RNA core as substrate
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.16.1
acetylation
complete inhibition by acetylation of the 2'-OH of tRNA
Bos taurus
3.1.16.1
arsenite
weak inhibitor with RNA core as substrate
Bos taurus
3.1.16.1
Cu2+
-
Bos taurus
3.1.16.1
Deoxyribonucleoside 3'-phosphates
-
Bos taurus
3.1.16.1
F-
weak inhibitor with RNA core as substrate
Bos taurus
3.1.16.1
glycosylation
no activity with glycosylated oligonucleotides obtained from T4 phage DNA
Bos taurus
3.1.16.1
Hg2+
with RNA core as substrate
Bos taurus
3.1.16.1
Mg2+
with RNA core as substrate
Bos taurus
3.1.16.1
Mn2+
with RNA core as substrate
Bos taurus
3.1.16.1
Zn2+
with RNA core as substrate
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.16.1
spleen
-
Bos taurus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.16.1
-15C, in 50% glycerol
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.16.1
4-nitrophenyl phosphate
-
134135
Bos taurus
4-nitrophenol + phosphate
-
134135
Bos taurus
?
3.1.16.1
bis-(4-nitrophenyl)phosphate + H2O
-
134135
Bos taurus
4-nitrophenyl phosphate + 4-nitrophenol
-
134135
Bos taurus
?
3.1.16.1
DNA + H2O
denatured only, no native or double stranded
134135
Bos taurus
deoxyribonucleoside 3'-phosphates
-
134135
Bos taurus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.16.1
5.8
-
substrate bis-(p-nitrophenyl) phosphate in 0.25 M succinate buffer
Bos taurus