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Literature summary extracted from

  • Dolapchiev, L.B.; Bakalova, A.
    Simple purification and some properties of beef spleen exonuclease (1982), Prep. Biochem., 12, 121-136.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.16.1 analysis kinetic studies on macromolecular substrates degradation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.16.1 Urea after 2 h incubation almost 80% of activity with 1.0 M, 40% with 2.0 M, no activity with 5.0 M Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.16.1 98000
-
molecular sieving, SDS gel electrophoresis, sucrose density gradient centrifugation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.16.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.16.1 no endonuclease and phophomonoesterase activities Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.16.1 spleen
-
Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
3.1.16.1 4°C, lyophilized crude enzyme, more than 1 year, no loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.16.1 RNA core + H2O
-
Bos taurus nucleoside 3'-phosphates
-
?
3.1.16.1 thymidine 3'-(4-nitrophenyl)phosphate + H2O
-
Bos taurus thymidine 3'-phosphate + 4-nitrophenol
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.16.1 phosphodiesterase II
-
Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.16.1 35 60 incubation at pH 5.7, 10 min: 90% of initial activity at 35°C, 10% at 60°C Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.16.1 5 7 incubation of pure enzyme for 2 h at 4°C, beyond the limits sharp decrease of stability Bos taurus