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Literature summary extracted from

  • Ohtaka, Y.; Uchida, K.; Sakai, T.
    Purification and properties of ribonuclease from yeast (1963), J. Biochem., 54, 322-327.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.14.1 EDTA slight activation Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.14.1 Cd2+ 100% inhibition by 0.01 M CdCl2 Saccharomyces cerevisiae
3.1.14.1 Co2+ 100% inhibition by 0.01 M CoCl2 Saccharomyces cerevisiae
3.1.14.1 Cu2+ 50% inhibition by 1 mM CuSO4 Saccharomyces cerevisiae
3.1.14.1 glutathione 95% inhibition by 0.01 M Saccharomyces cerevisiae
3.1.14.1 Mn2+ 20% of maximal activity Saccharomyces cerevisiae
3.1.14.1 Zn2+ 100% inhibition by 0.1 mM ZnSO4, reversible by addition of 1 mM EDTA, sodium citrate and histidine, not by glycine, arginine, agmatine and sodium acetate even at 0.01 M Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.14.1 KCl slight activation Saccharomyces cerevisiae
3.1.14.1 KH2PO4 10-80% activation at various concentrations Saccharomyces cerevisiae
3.1.14.1 Na2SO4 slight activation Saccharomyces cerevisiae
3.1.14.1 NaCl slight activation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.14.1 ribonucleic acid + H2O Saccharomyces cerevisiae exonucleolytic cleavage 3'-phosphomononucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.14.1 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.14.1
-
 Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.14.1 exonucleolytic cleavage to nucleoside 3'-phosphates exonucleolytic cleavage Saccharomyces cerevisiae
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.14.1 additional information released from pressed cells Saccharomyces cerevisiae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.14.1 additional information
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
3.1.14.1 4°C, under toluene, pH 7.5, several weeks, little loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.14.1 additional information not: DNA, diphenylphosphate, 2',3'-cyclic nucleotides Saccharomyces cerevisiae ?
-
 ?
3.1.14.1 ribonucleic acid + H2O exonucleolytic cleavage Saccharomyces cerevisiae 3'-phosphomononucleotides
-
 ?
3.1.14.1 ribonucleic acid + H2O exonucleolytic cleavage Saccharomyces cerevisiae 3'-phosphomononucleotides 3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.14.1 50
-
 30 min reaction time Saccharomyces cerevisiae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.14.1 35 65 less than 15% of maximum activity above and below Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.14.1 40
-
 several h under toluene, pH 7.5 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.14.1 7.5
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.14.1 4.5 9 Ph 4.5: about 15% of maximum activity, pH 9.0: about 50% of maximum activity Saccharomyces cerevisiae