EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.1 | 2-mercaptoethanol | - |
Rattus norvegicus | |
3.1.4.1 | 5'-nucleotides | - |
Rattus norvegicus | |
3.1.4.1 | ADP | - |
Rattus norvegicus | |
3.1.4.1 | AMP | 5'-AMP and slightly 3'-AMP | Rattus norvegicus | |
3.1.4.1 | ATP | - |
Rattus norvegicus | |
3.1.4.1 | Cu2+ | - |
Rattus norvegicus | |
3.1.4.1 | CuCl2 | - |
Rattus norvegicus | |
3.1.4.1 | EDTA | - |
Rattus norvegicus | |
3.1.4.1 | Hg2+ | - |
Rattus norvegicus | |
3.1.4.1 | HgCl2 | - |
Rattus norvegicus | |
3.1.4.1 | NAD+ | competitive | Rattus norvegicus | |
3.1.4.1 | nucleoside 5'monophosphate | - |
Rattus norvegicus | |
3.1.4.1 | nucleoside-5'-diphosphate | - |
Rattus norvegicus | |
3.1.4.1 | p-chloromercuribenzoate | slight inhibition | Rattus norvegicus | |
3.1.4.1 | RNA | competitive | Rattus norvegicus | |
3.1.4.1 | terminal 3'-monophosphoryl group of substrates | - |
Rattus norvegicus | |
3.1.4.1 | UDP | - |
Rattus norvegicus | |
3.1.4.1 | UMP | 5'-UMP and very slightly 3'-UMP | Rattus norvegicus | |
3.1.4.1 | UTP | - |
Rattus norvegicus | |
3.1.13.3 | 3'-AMP | slight | Rattus norvegicus | |
3.1.13.3 | 5'-AMP | - |
Rattus norvegicus | |
3.1.13.3 | 5'-UMP | - |
Rattus norvegicus | |
3.1.13.3 | ADP | - |
Rattus norvegicus | |
3.1.13.3 | ATP | - |
Rattus norvegicus | |
3.1.13.3 | beta-mercaptoethanol | slight | Rattus norvegicus | |
3.1.13.3 | CuCl2 | slight | Rattus norvegicus | |
3.1.13.3 | EDTA | - |
Rattus norvegicus | |
3.1.13.3 | HgCl2 | - |
Rattus norvegicus | |
3.1.13.3 | p-chloromercuribenzoate | slight | Rattus norvegicus | |
3.1.13.3 | UDP | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.1 | 0.41 | - |
4-nitrophenyl uridine 5'-phosphate | - |
Rattus norvegicus | |
3.1.13.3 | 0.41 | - |
p-nitrophenyl uridine 5'-monophosphate | - |
Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.1 | Ca2+ | activation | Rattus norvegicus | |
3.1.4.1 | Co2+ | slight activation | Rattus norvegicus | |
3.1.4.1 | divalent cations | activation | Rattus norvegicus | |
3.1.4.1 | K+ | activation with high concentration | Rattus norvegicus | |
3.1.4.1 | KCl | activation | Rattus norvegicus | |
3.1.4.1 | Mg2+ | activation | Rattus norvegicus | |
3.1.4.1 | monovalent cations | activation with high concentrations | Rattus norvegicus | |
3.1.4.1 | Na+ | activation with high concentration | Rattus norvegicus | |
3.1.4.1 | NaCl | activation | Rattus norvegicus | |
3.1.13.3 | Ca2+ | activation | Rattus norvegicus | |
3.1.13.3 | Co2+ | activation | Rattus norvegicus | |
3.1.13.3 | K+ | activates at high concentration | Rattus norvegicus | |
3.1.13.3 | Mg2+ | activation | Rattus norvegicus | |
3.1.13.3 | Na+ | activates at high concentration | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.13.3 | oligonucleotides + H2O | Rattus norvegicus | - |
nucleoside 5'-phosphates | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.1 | Rattus norvegicus | - |
- |
- |
3.1.13.3 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.1 | partial | Rattus norvegicus |
3.1.13.3 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.4.1 | liver | - |
Rattus norvegicus | - |
3.1.13.3 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.4.1 | 0.48 | - |
substrate: NAD+ | Rattus norvegicus |
3.1.4.1 | 1.2 | - |
substrate: p-nitrophenyluridine 5'-phosphate | Rattus norvegicus |
3.1.4.1 | 1.88 | - |
substrate: ApU | Rattus norvegicus |
3.1.13.3 | 1.2 | - |
p-nitrophenyl uridine 5'-monophosphate | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.1 | 4-nitrophenyl uridine 5'-phosphate + H2O | - |
Rattus norvegicus | 4-nitrophenol + uridine 5'-phosphate | - |
? | |
3.1.4.1 | 5'-p-nitrophenyl-2',3'-isopropyl-5'-UMP + H2O | - |
Rattus norvegicus | ? | - |
? | |
3.1.4.1 | 5'-p-nitrophenylnucleoside 5'-monophosphate + H2O | - |
Rattus norvegicus | nucleoside 5'-monophosphate + p-nitrophenol | - |
? | |
3.1.4.1 | 5'-p-nitrophenyluridine 5'-phosphate + H2O | - |
Rattus norvegicus | 5'-UMP + p-nitrophenol | - |
? | |
3.1.4.1 | dinucleoside monophosphates + H2O | - |
Rattus norvegicus | mononucleoside 5'-phosphate | - |
? | |
3.1.4.1 | DNA + H2O | heat-denatured: very slowly, native DNA is not hydrolyzed | Rattus norvegicus | DNA(n-1) + 2'-deoxynucleoside 5'-phosphate | - |
? | |
3.1.4.1 | additional information | no activity with dinucleotides bearing 3'-phosphate | Rattus norvegicus | ? | - |
? | |
3.1.4.1 | additional information | enzyme does not have a strict base specificity, it does not attack oligonucleotides with a 3'-phosphate terminal | Rattus norvegicus | ? | - |
? | |
3.1.4.1 | additional information | no activity with UTP and UDP | Rattus norvegicus | ? | - |
? | |
3.1.4.1 | NAD+ + H2O | - |
Rattus norvegicus | 5'-AMP + NMN | - |
? | |
3.1.4.1 | oligonucleotides + H2O | specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner | Rattus norvegicus | oligonucleotides(n-1) + 5'-mononucleotides | - |
? | |
3.1.4.1 | polymer synthesized from NAD+ + H2O | chain length 27-30 | Rattus norvegicus | ? | - |
? | |
3.1.4.1 | polynucleotides + H2O | very slowly | Rattus norvegicus | polynucleotides(n-1) + 5'-mononucleotides | - |
? | |
3.1.4.1 | RNA + H2O | very slowly | Rattus norvegicus | RNA(n-1) + nucleoside 5'-phosphate | - |
? | |
3.1.13.3 | DNA + H2O | hydrolyzed very slowly, not: ADP, UDP, ATP, UTP | Rattus norvegicus | nucleoside 5'-phosphates | - |
? | |
3.1.13.3 | NAD+ + H2O | - |
Rattus norvegicus | NMN + AMP | - |
? | |
3.1.13.3 | oligonucleotides + H2O | - |
Rattus norvegicus | nucleoside 5'-phosphates | - |
? | |
3.1.13.3 | oligonucleotides + H2O | no base specificity | Rattus norvegicus | nucleoside 5'-phosphates | - |
? | |
3.1.13.3 | oligonucleotides + H2O | initial site of attack: 3'-hydroxyl end | Rattus norvegicus | nucleoside 5'-phosphates | - |
? | |
3.1.13.3 | p-nitrophenyl uridine 5'-monophosphate + H2O | - |
Rattus norvegicus | p-nitrophenol + 5'-UMP | - |
? | |
3.1.13.3 | p-nitrophenyl-2',3'-isopropyl-5'-UMP + H2O | - |
Rattus norvegicus | p-nitrophenol + isopropyl-5'-UMP | - |
? | |
3.1.13.3 | polymer synthesized from NAD+ + H2O | chain length 27-30 | Rattus norvegicus | ? | - |
? | |
3.1.13.3 | RNA + H2O | hydrolyzed very slowly | Rattus norvegicus | nucleoside 5'-phosphates | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.4.1 | additional information | - |
enzyme is heat labile | Rattus norvegicus |
3.1.4.1 | 50 | - |
below, enzyme is heat labile | Rattus norvegicus |
3.1.4.1 | 70 | - |
pH 7.3, 5 min, complete loss of activity | Rattus norvegicus |
3.1.13.3 | 70 | - |
pH 7.3, 5 min, complete loss of activity | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.1 | 10 | 10.5 | - |
Rattus norvegicus |
3.1.13.3 | 10.1 | - |
nitrophenyl uridine 5'-monophosphate | Rattus norvegicus |
3.1.13.3 | 10.5 | - |
NAD | Rattus norvegicus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.1 | 8 | 11 | - |
Rattus norvegicus |
3.1.13.3 | 8 | 10 | nitrophenyl uridine 5'-monphosphate, pH 8: about 15% of maximum activity, pH 10: about 10% of maximum activity | Rattus norvegicus |