Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wei, X.; Peterson, D.L.
    Expression, purification and characterization of an active RNase H domain of the Hepatitis B viral polymerase (1996), J. Biol. Chem., 271, 32617-32622.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.13.2 KCl maximal enzyme activity at 0-20 mM Hepatitis B virus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.7 RNase H domain Hepatitis B virus
3.1.13.2 RNase H domain Hepatitis B virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 KCl inhibition above 20 mM Hepatitis B virus
3.1.13.2 KCl at concentrations greater than 20 mM Hepatitis B virus
3.1.13.2 Mg2+ inhibitory at concentrations greater than 10 mM Hepatitis B virus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 Mg2+ enzyme prefers Mg2+ over Mn2+ Hepatitis B virus
2.7.7.7 Mn2+ enzyme prefers Mg2+ over Mn2+ Hepatitis B virus
3.1.13.2 Mg2+ maximum activity at 2mM Hepatitis B virus
3.1.13.2 Mg2+ preference for Mg2+ to Mn2+ Hepatitis B virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 19960
-
 calculated from amino acid composition, RNase H domain Hepatitis B virus
2.7.7.7 20000
-
 RNase H domain, SDS-PAGE Hepatitis B virus
3.1.13.2 20000
-
 Ni-Sepharose column chromatography, amino acid calculation Hepatitis B virus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn Hepatitis B virus
-
 diphosphate + DNAn+1
-
 ?
3.1.13.2 DNA-RNA hybrid duplex + H2O Hepatitis B virus
-
 oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
-
 ?
3.1.13.2 DNA-RNA hybrid duplex + H2O Hepatitis B virus HBV
-
 oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Hepatitis B virus P03156
-
-
3.1.13.2 Hepatitis B virus
-
 HBV
-
3.1.13.2 Hepatitis B virus HBV
-
 HBV
-
3.1.13.2 More
-
 retrovirus: RNaseH and transcriptase are present in a single polypeptide
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.7.7.7 sensitive to oxidation, enzyme can be stabilized by addition of dithiothreitol or 2-mercaptoethanol Hepatitis B virus
3.1.13.2 easily inactivated by oxidation, no activity detected in absence of dithiothreitol or mercaptoethanol Hepatitis B virus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7 RNase H domain Hepatitis B virus
3.1.13.2 RNase H domain Hepatitis B virus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.13.2
-
 Hepatitis B virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Hepatitis B virus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn RNase H domain degrades RNA component of RNA-DNA hybrids Hepatitis B virus diphosphate + DNAn+1
-
 ?
3.1.13.2 DNA-RNA hybrid duplex + H2O
-
 Hepatitis B virus oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
-
 ?
3.1.13.2 DNA-RNA hybrid duplex + H2O poor substrate: poly(rG)-poly(dC) Hepatitis B virus oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
-
 ?
3.1.13.2 DNA-RNA hybrid duplex + H2O
-
 Hepatitis B virus HBV oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
-
 ?
3.1.13.2 DNA-RNA hybrid duplex + H2O poor substrate: poly(rG)-poly(dC) Hepatitis B virus HBV oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.7 60
-
 stable for 10 min, RNase H activity Hepatitis B virus
2.7.7.7 100
-
 no RNase H activity after 10 min Hepatitis B virus
3.1.13.2 60
-
 for 10 min, does not affect the reaction Hepatitis B virus
3.1.13.2 100
-
 for 10 min, no activity remains Hepatitis B virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 8 8.5
-
 Hepatitis B virus
3.1.13.2 8 8.5
-
 Hepatitis B virus