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Literature summary extracted from
- Purification of a human polyribosome-associated 3'-5'exoribonuclease (1994), J. Biol. Chem., 269, 31814-31821.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.13.1 | additional information | not inhibited by RNase A-type inhibitor | Homo sapiens | |
| 3.1.13.1 | poly(A) binding protein | inhibits degradation of poly(A)+-strands | Homo sapiens | |
| 3.1.13.1 | RNA with a terminal 3'-phosphate group | on the potential substrate molecule | Homo sapiens |  |
| 3.1.13.1 | ss DNA oligonucleotides | - |
Homo sapiens |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.13.1 | polysome | - |
Homo sapiens | 5844 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.13.1 | Mg2+ | necessary for activity | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.13.1 | 33000 | - |
SDS-PAGE | Homo sapiens |
| 3.1.13.1 | 37000 | - |
native state gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.13.1 | ss RNA + H2O | Homo sapiens | - |
5'-phosphomononucleotides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.13.1 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.13.1 | 5300-26500fold, to homogeneity | Homo sapiens |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.1.13.1 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.13.1 | erythroleukemia cell | - |
Homo sapiens | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.13.1 | 4°C, 10-ml fractions containing 1 mg trypsin inhibitor carrier protein because RNase II loses activity under this conditions at protein concentrations below 0.1 mg/ml | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.13.1 | duplex RNA + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.1.13.1 | additional information | 3'-phosphorylated RNA is not hydrolyzed | Homo sapiens | ? | - |
? | |
| 3.1.13.1 | mRNA + H2O | - |
Homo sapiens | 5'-phosphomononucleotides | - |
? | |
| 3.1.13.1 | poly(A) + H2O | - |
Homo sapiens | 5'-AMP + oligonucleotide | - |
? | |
| 3.1.13.1 | ss RNA + H2O | - |
Homo sapiens | 5'-phosphomononucleotides | - |
? | |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.13.1 | 7 | 8 | - |
Homo sapiens |
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