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Literature summary extracted from
- Overexpression, purification, and properties of Escherichia coli ribonuclease II (1996), J. Biol. Chem., 271, 1048-1053.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.6.1.19 | overexpression in Escherichia coli | Escherichia coli |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.6.1.19 | substrate and product stalilizes | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.13.1 | Ca2+ | - |
Escherichia coli |  |
| 3.1.13.1 | ds plasmid DNA | - |
Escherichia coli | |
| 3.1.13.1 | ss DNA oligonucleotides | - |
Escherichia coli |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.6.1.19 | KCl | enhances activity | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.13.1 | 70000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.13.1 | ss RNA + H2O | Escherichia coli | - |
5'-phosphomononucleotides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.13.1 | Escherichia coli | - |
- |
- |
| 4.6.1.19 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.13.1 | - |
Escherichia coli |
| 4.6.1.19 | recombinant protein | Escherichia coli |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.1.13.1 | Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates | acts in a processive manner | Escherichia coli |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.1.13.1 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.13.1 | 68.3 | - |
- |
Escherichia coli |
| 4.6.1.19 | 223500 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.13.1 | ss RNA + H2O | - |
Escherichia coli | 5'-phosphomononucleotides | - |
? | |
| 4.6.1.19 | RNA + H2O | hydrolyzes in 3'- to 5'-direction, hydrolyzes single stranded RNA | Escherichia coli | 3'-phosphomononucleotides | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.13.1 | 37 | - |
for 5 min, in absence of substrate, less than 1% of activity remains | Escherichia coli |
| 3.1.13.1 | 37 | - |
in presence of substrate, product or deoxy-oligonucleotides (dC)27: active up to 60 min | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.6.1.19 | 0.15 | - |
RNA | - |
Escherichia coli | |
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