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Literature summary extracted from

  • Kumagai, H.; Igarshi, K.; Tanaka, K.; Nakao, H.; Hirose, S.
    A microsomal exoribonuclease from rat liver (1979), Biochim. Biophys. Acta, 566, 192-199.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.13.1 spermine activates in absence of K+ the poly(C)- and poly(A)-breakdown Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.13.1 monovalent or divalent cations high concentrations; Mg2+-concentrations above 2 mM Rattus norvegicus
3.1.13.1 p-chloromercuribenzoate
-
 Rattus norvegicus
3.1.13.1 Poly(G)
-
 Rattus norvegicus
3.1.13.1 spermine slight inhibition of poly(U)-degradation Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.13.1 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.13.1 K+ optimum for poly(C) as substrate: 25 mM Rattus norvegicus
3.1.13.1 K+ optimum for poly(A) as substrate: 100 mM Rattus norvegicus
3.1.13.1 K+ necessary for activity Rattus norvegicus
3.1.13.1 Mg2+ necessary for activity Rattus norvegicus
3.1.13.1 Mg2+ optimum: 1-2 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.13.1 80000
-
 SDS-PAGE Rattus norvegicus
3.1.13.1 83000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.13.1 ss RNA + H2O Rattus norvegicus
-
 5'-phosphomononucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.13.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.13.1 100fold Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.13.1 Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates acts in a processive manner Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.13.1 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.13.1 additional information poly(G) is not hydrolyzed Rattus norvegicus ?
-
 ?
3.1.13.1 poly(A) + H2O
-
 Rattus norvegicus 5'-AMP + oligonucleotide
-
 ?
3.1.13.1 poly(C) + H2O
-
 Rattus norvegicus 5'-CMP + oligonucleotide
-
 ?
3.1.13.1 poly(U) + H2O
-
 Rattus norvegicus 5'-UMP + oligonucleotide
-
 ?
3.1.13.1 ss RNA + H2O
-
 Rattus norvegicus 5'-phosphomononucleotides
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.13.1 90
-
 for 1 min, no activity remains Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.13.1 7.5 8
-
 Rattus norvegicus