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Literature summary extracted from
- An Escherichia coli ribonuclease which removes an extra nucleotide from a biosynthetic intermediate of bacteriophage T4 proline transfer RNA (1978), Nucleic Acids Res., 5, 4129-4139.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.13.1 | monovalent or divalent cations | 10 mM of Mg2+ reduce activity 2fold, activity barely detectable at 20 mM Mg2+; high concentrations | Escherichia coli |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.13.1 | cytosol | - |
Escherichia coli | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.13.1 | Mg2+ | necessary for activity | Escherichia coli |  |
| 3.1.13.1 | Mg2+ | optimum: 2 mM | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.13.1 | 35000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.13.1 | precursor tRNA + H2O | Escherichia coli | - |
mature tRNA + 5'-phosphomononucleotides | - |
? | |
| 3.1.13.1 | ss RNA + H2O | Escherichia coli | - |
5'-phosphomononucleotides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.13.1 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.13.1 | partial purification, between 50-100fold | Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.13.1 | -70°C, stable for at least 1 month without loss of activity | Escherichia coli |
| 3.1.13.1 | 0°C, half-life of 5-7 days | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.13.1 | precursor tRNA + H2O | - |
Escherichia coli | mature tRNA + 5'-phosphomononucleotides | - |
? | |
| 3.1.13.1 | ss RNA + H2O | - |
Escherichia coli | 5'-phosphomononucleotides | - |
? | |
| 3.1.13.1 | tRNA + H2O | RNase BN | Escherichia coli | ? | - |
? | |
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Release 2023.1 (January 2023)
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