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Literature summary extracted from

  • Nossal, N.G.; Singer, M.F.
    The processive degradation of individual polyribonucleotide chains. I. Escherichia coli ribonuclease II (1968), J. Biol. Chem., 243, 913-922.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.13.1 K+ activating Escherichia coli
3.1.13.1 Mg2+ necessary for activity Escherichia coli
3.1.13.1 NH4+ activating Escherichia coli
3.1.13.1 NH4+ can substitute for K+ to a small extend Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.13.1 65000
-
sucrose density gradient centrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.13.1 ss RNA + H2O Escherichia coli
-
5'-phosphomononucleotides
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.13.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.13.1
-
Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.13.1 Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates acts in a processive manner Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.13.1 additional information helical RNA is not hydrolyzed Escherichia coli ?
-
?
3.1.13.1 poly(A) + H2O
-
Escherichia coli 5'-AMP + oligonucleotide
-
?
3.1.13.1 ss RNA + H2O
-
Escherichia coli 5'-phosphomononucleotides
-
?
3.1.13.1 ss RNA oligonucleotides with chain lengths less than seven + H2O at high concentrations Escherichia coli 5'-phosphomononucleotides
-
?
3.1.13.1 tRNA + H2O poor substrate Escherichia coli ?
-
?