Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Oishi, M.
    An ATP-dependent deoxyribonuclease from Escherichia coli with a possible role in genetic recombination (1969), Proc. Natl. Acad. Sci. USA, 64, 1292-1299.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.11.5 3',5'-cyclic AMP less effective than ATP Escherichia coli
3.1.11.5 5'-AMP less effective than ATP Escherichia coli
3.1.11.5 adenine less effective than ATP Escherichia coli
3.1.11.5 adenosine less effective than ATP Escherichia coli
3.1.11.5 ADP less effective than ATP Escherichia coli
3.1.11.5 CTP less effective than ATP Escherichia coli
3.1.11.5 dCTP less effective than ATP Escherichia coli
3.1.11.5 GTP less effective than ATP Escherichia coli
3.1.11.5 S-adenosylmethionine less effective than ATP Escherichia coli
3.1.11.5 TTP less effective than ATP Escherichia coli
3.1.11.5 UTP less effective than ATP Escherichia coli

Application

EC Number Application Comment Organism
3.1.11.5 analysis possible role in genetic recombination Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.11.5 0.043
-
ATP
-
Escherichia coli
3.1.11.5 0.048
-
dATP
-
Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.11.5 Mg2+
-
Escherichia coli
3.1.11.5 Mn2+ less effective than Mg2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.11.5 350000
-
sedimentation, calculated Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.5 double-stranded DNA + H2O Escherichia coli responsible for several steps in genetic recombination process single-stranded DNA fragments
-
?
3.1.11.5 double-stranded DNA + H2O Escherichia coli ATP-dependent exonuclease single-stranded DNA fragments
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.5 Escherichia coli
-
mutants lacking the recB or recC or recD gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.11.5 partial Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.11.5 0.024
-
-
Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.1.11.5 0°C, Tris, MgCl2, EDTA, mercaptoethanol, 1 d, 5-10% loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.5 ATP + H2O
-
Escherichia coli ADP + phosphate
-
?
3.1.11.5 double-stranded DNA + H2O acts on native, heat-denatured and glucosylated DNA Escherichia coli single-stranded DNA fragments
-
?
3.1.11.5 double-stranded DNA + H2O responsible for several steps in genetic recombination process Escherichia coli single-stranded DNA fragments
-
?
3.1.11.5 double-stranded DNA + H2O ATP-dependent exonuclease Escherichia coli single-stranded DNA fragments
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.11.5 37
-
assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.11.5 7.5 9.5
-
Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.11.5 7.5 9.5
-
Escherichia coli