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Literature summary extracted from

  • Telander Muskavitch, K.M.; Linn, S.
    recBC-like enzymes:exonuclease V deoxyribonucleases (1981), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 14, 233-250.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.11.5 dATP nearly as effective as ATP Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.11.5 E. coli Bacteriophage proteins
-
 Escherichia coli
3.1.11.5 E. coli single stranded DNA binding protein SSB Escherichia coli
3.1.11.5 pyridoxal 5'-phosphate
-
 Escherichia coli
3.1.11.5 small DNA fragments
-
 Bacillus subtilis
3.1.11.5 thermostable protein
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.11.5 Mg2+ required, optimal concentration around 3-12 mM Alcaligenes faecalis
3.1.11.5 Mg2+ required, optimal concentration around 3-12 mM Haemophilus influenzae
3.1.11.5 Mg2+ required, optimal concentration around 3-12 mM Bacillus subtilis
3.1.11.5 Mg2+ required, optimal concentration around 3-12 mM Escherichia coli
3.1.11.5 Mg2+ required, optimal concentration around 3-12 mM Mycolicibacterium smegmatis
3.1.11.5 Mg2+ required, optimal concentration around 3-12 mM Pseudomonas aeruginosa
3.1.11.5 Mg2+ required, optimal concentration around 3-12 mM Streptococcus pneumoniae
3.1.11.5 Mg2+ required, optimal concentration around 3-12 mM Bacillus cereus
3.1.11.5 Mg2+ required, optimal concentration around 3-12 mM Micrococcus luteus
3.1.11.5 Mg2+ required, optimal concentration around 3-12 mM Brevibacillus laterosporus
3.1.11.5 Mn2+ less effective than Mg2+ Alcaligenes faecalis
3.1.11.5 Mn2+ less effective than Mg2+ Haemophilus influenzae
3.1.11.5 Mn2+ less effective than Mg2+ Bacillus subtilis
3.1.11.5 Mn2+ less effective than Mg2+ Escherichia coli
3.1.11.5 Mn2+ less effective than Mg2+ Mycolicibacterium smegmatis
3.1.11.5 Mn2+ less effective than Mg2+ Pseudomonas aeruginosa
3.1.11.5 Mn2+ less effective than Mg2+ Streptococcus pneumoniae
3.1.11.5 Mn2+ less effective than Mg2+ Bacillus cereus
3.1.11.5 Mn2+ less effective than Mg2+ Micrococcus luteus
3.1.11.5 Mn2+ less effective than Mg2+ Brevibacillus laterosporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.11.5 42500
-
 1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
3.1.11.5 52500
-
 1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
3.1.11.5 60000
-
 1 * 170000 + 1 * 60000, glycerol gradient sedimentation Escherichia coli
3.1.11.5 62000
-
 1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
3.1.11.5 68000
-
 1 * 115000 + 1 * 107000 + 1 * 68000 Haemophilus influenzae
3.1.11.5 70000
-
 1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
3.1.11.5 81000
-
 1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
3.1.11.5 107000
-
 1 * 115000 + 1 * 107000 + 1 * 68000 Haemophilus influenzae
3.1.11.5 115000
-
 1 * 115000 + 1 * 107000 + 1 * 68000 Haemophilus influenzae
3.1.11.5 128000
-
 1 * 140000 + 1 * 128000, dissociation by boiling, SDS-PAGE Escherichia coli
3.1.11.5 140000
-
 1 * 140000 + 1 * 128000, dissociation by boiling, SDS-PAGE Escherichia coli
3.1.11.5 140000
-
 1 * 155000 + 1 * 140000, protease inhibitor phenylmethylsulfonyl flouride was added Bacillus subtilis
3.1.11.5 155000
-
 1 * 155000 + 1 * 140000, protease inhibitor phenylmethylsulfonyl flouride was added Bacillus subtilis
3.1.11.5 170000
-
 1 * 170000 + 1 * 60000, glycerol gradient sedimentation Escherichia coli
3.1.11.5 270000
-
-
 Bacillus subtilis
3.1.11.5 270000
-
-
 Escherichia coli
3.1.11.5 290000
-
-
 Haemophilus influenzae
3.1.11.5 300000
-
-
 Pseudomonas aeruginosa
3.1.11.5 308000
-
-
 Bacillus subtilis
3.1.11.5 350000
-
-
 Escherichia coli
3.1.11.5 450000
-
-
 Strongylocentrotus intermedius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.5 ATP + H2O Alcaligenes faecalis
-
 ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O Haemophilus influenzae
-
 ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O Bacillus subtilis
-
 ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O Mycolicibacterium smegmatis
-
 ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O Pseudomonas aeruginosa
-
 ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O Streptococcus pneumoniae
-
 ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O Bacillus cereus
-
 ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O Micrococcus luteus
-
 ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O Brevibacillus laterosporus
-
 ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O Strongylocentrotus intermedius
-
 ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O Escherichia coli DNA dependent ADP + phosphate
-
 ?
3.1.11.5 double-stranded DNA + H2O Alcaligenes faecalis
-
 single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O Haemophilus influenzae
-
 single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O Bacillus subtilis
-
 single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O Mycolicibacterium smegmatis
-
 single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O Pseudomonas aeruginosa
-
 single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O Streptococcus pneumoniae
-
 single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O Bacillus cereus
-
 single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O Micrococcus luteus
-
 single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O Brevibacillus laterosporus
-
 single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O Strongylocentrotus intermedius
-
 single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O Escherichia coli ATP-dependent exonuclease single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O Escherichia coli ATP-dependent helicase single-stranded DNA fragments
-
 ?
3.1.11.5 single stranded DNA + H2O Alcaligenes faecalis
-
 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O Haemophilus influenzae
-
 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O Mycolicibacterium smegmatis
-
 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O Streptococcus pneumoniae
-
 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O Bacillus cereus
-
 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O Micrococcus luteus
-
 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O Brevibacillus laterosporus
-
 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O Escherichia coli ATP-dependent exonuclease 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O Escherichia coli ATP-dependent endonuclease 5'-phosphomonoester oligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.5 Alcaligenes faecalis
-
-
-
3.1.11.5 Bacillus cereus
-
-
-
3.1.11.5 Bacillus subtilis
-
-
-
3.1.11.5 Brevibacillus laterosporus
-
-
-
3.1.11.5 Escherichia coli
-
 mutants lacking the recB or recC or recD gene
-
3.1.11.5 Haemophilus influenzae
-
-
-
3.1.11.5 Micrococcus luteus
-
 Micrococcus luteus
-
3.1.11.5 Mycolicibacterium smegmatis
-
-
-
3.1.11.5 Pseudomonas aeruginosa
-
-
-
3.1.11.5 Streptococcus pneumoniae
-
-
-
3.1.11.5 Strongylocentrotus intermedius
-
 sea urchin
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.11.5 Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides preference for double-stranded DNA, possesses DNA-dependent ATPase activity, acts endonucleolytically on single-stranded circular DNA Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.11.5 0.7
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.5 ATP + H2O
-
 Alcaligenes faecalis ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O
-
 Haemophilus influenzae ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O
-
 Bacillus subtilis ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O
-
 Mycolicibacterium smegmatis ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O
-
 Pseudomonas aeruginosa ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O
-
 Streptococcus pneumoniae ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O
-
 Bacillus cereus ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O
-
 Micrococcus luteus ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O
-
 Brevibacillus laterosporus ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O
-
 Strongylocentrotus intermedius ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O linear DNA dependent Alcaligenes faecalis ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O linear DNA dependent Haemophilus influenzae ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O linear DNA dependent Bacillus subtilis ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O linear DNA dependent Escherichia coli ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O linear DNA dependent Mycolicibacterium smegmatis ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O linear DNA dependent Pseudomonas aeruginosa ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O linear DNA dependent Streptococcus pneumoniae ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O linear DNA dependent Bacillus cereus ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O linear DNA dependent Micrococcus luteus ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O linear DNA dependent Brevibacillus laterosporus ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O linear DNA dependent Strongylocentrotus intermedius ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O complete inhibition of DNAse activity has no effect on ATPase activity Escherichia coli ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O DNA dependent Escherichia coli ADP + phosphate
-
 ?
3.1.11.5 double-stranded DNA at presence of low Mg2+ Haemophilus influenzae intermediates with single stranded regions
-
 ?
3.1.11.5 double-stranded DNA unwinding in the presence of E. coli binding protein SSB or high levels of ATP Escherichia coli intermediates with single stranded regions
-
 ?
3.1.11.5 double-stranded DNA + H2O
-
 Alcaligenes faecalis single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O
-
 Haemophilus influenzae single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O
-
 Bacillus subtilis single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O
-
 Mycolicibacterium smegmatis single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O
-
 Pseudomonas aeruginosa single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O
-
 Streptococcus pneumoniae single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O
-
 Bacillus cereus single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O
-
 Micrococcus luteus single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O
-
 Brevibacillus laterosporus single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O
-
 Strongylocentrotus intermedius single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O no activity on circular DNA Haemophilus influenzae single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O no activity on circular DNA Bacillus subtilis single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O no activity on circular DNA Escherichia coli single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O no activity on circular DNA Micrococcus luteus single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP dependent double-stranded DNA exonuclease Alcaligenes faecalis single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP dependent double-stranded DNA exonuclease Haemophilus influenzae single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP dependent double-stranded DNA exonuclease Bacillus subtilis single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP dependent double-stranded DNA exonuclease Escherichia coli single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP dependent double-stranded DNA exonuclease Mycolicibacterium smegmatis single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP dependent double-stranded DNA exonuclease Pseudomonas aeruginosa single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP dependent double-stranded DNA exonuclease Streptococcus pneumoniae single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP dependent double-stranded DNA exonuclease Bacillus cereus single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP dependent double-stranded DNA exonuclease Micrococcus luteus single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP dependent double-stranded DNA exonuclease Brevibacillus laterosporus single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP dependent double-stranded DNA exonuclease Strongylocentrotus intermedius single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP-dependent exonuclease Escherichia coli single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP-dependent helicase Escherichia coli single-stranded DNA fragments
-
 ?
3.1.11.5 single stranded DNA + H2O
-
 Alcaligenes faecalis 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O
-
 Haemophilus influenzae 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O
-
 Mycolicibacterium smegmatis 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O
-
 Streptococcus pneumoniae 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O
-
 Bacillus cereus 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O
-
 Micrococcus luteus 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O
-
 Brevibacillus laterosporus 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O also endonuclease activity on circular DNA Haemophilus influenzae 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O also endonuclease activity on circular DNA Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP dependence varies Alcaligenes faecalis 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP dependence varies Haemophilus influenzae 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP dependence varies Bacillus subtilis 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP dependence varies Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP dependence varies Mycolicibacterium smegmatis 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP dependence varies Streptococcus pneumoniae 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP dependence varies Bacillus cereus 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP dependence varies Micrococcus luteus 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP dependence varies Brevibacillus laterosporus 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O rate of degradation is less than double-stranded DNA Alcaligenes faecalis 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O rate of degradation is less than double-stranded DNA Haemophilus influenzae 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O rate of degradation is less than double-stranded DNA Bacillus subtilis 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O rate of degradation is less than double-stranded DNA Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O rate of degradation is less than double-stranded DNA Mycolicibacterium smegmatis 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O rate of degradation is less than double-stranded DNA Streptococcus pneumoniae 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O rate of degradation is less than double-stranded DNA Bacillus cereus 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O rate of degradation is less than double-stranded DNA Micrococcus luteus 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O rate of degradation is less than double-stranded DNA Brevibacillus laterosporus 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP-dependent exonuclease Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP-dependent endonuclease Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.11.5 dimer 1 * 140000 + 1 * 128000, dissociation by boiling, SDS-PAGE Escherichia coli
3.1.11.5 dimer 1 * 170000 + 1 * 60000, glycerol gradient sedimentation Escherichia coli
3.1.11.5 dimer 1 * 155000 + 1 * 140000, protease inhibitor phenylmethylsulfonyl flouride was added Bacillus subtilis
3.1.11.5 pentamer 1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
3.1.11.5 trimer
-
 Escherichia coli
3.1.11.5 trimer 1 * 115000 + 1 * 107000 + 1 * 68000 Haemophilus influenzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.11.5 9
-
-
 Alcaligenes faecalis
3.1.11.5 9
-
-
 Haemophilus influenzae
3.1.11.5 9
-
-
 Bacillus subtilis
3.1.11.5 9
-
-
 Escherichia coli
3.1.11.5 9
-
-
 Mycolicibacterium smegmatis
3.1.11.5 9
-
-
 Pseudomonas aeruginosa
3.1.11.5 9
-
-
 Streptococcus pneumoniae
3.1.11.5 9
-
-
 Bacillus cereus
3.1.11.5 9
-
-
 Micrococcus luteus
3.1.11.5 9
-
-
 Brevibacillus laterosporus