Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Little, J.W.; Lehman, I.R.; Kaiser, A.D.
    An exonuclease induced by bacteriophage lambda. I. Preparation of the crystalline enzyme (1967), J. Biol. Chem., 242, 672-678.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.11.3 by ammonium sulfate precepitation Escherichia coli
3.1.11.3 by ammonium sulfate precepitation Lambdavirus lambda

General Stability

EC Number General Stability Organism
3.1.11.3 repeated freezing and thawing causes loss of activity Escherichia coli
3.1.11.3 repeated freezing and thawing causes loss of activity Lambdavirus lambda

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.11.3 KCl 31% inhibition at 50 mM Escherichia coli
3.1.11.3 KCl 31% inhibition at 50 mM Lambdavirus lambda
3.1.11.3 NaCl 82% inhibition at 50 mM Escherichia coli
3.1.11.3 NaCl 82% inhibition at 50 mM Lambdavirus lambda
3.1.11.3 p-chloromercuribenzoate
-
 Escherichia coli
3.1.11.3 p-chloromercuribenzoate
-
 Lambdavirus lambda

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.11.3 Mg2+ required Escherichia coli
3.1.11.3 Mg2+ required Lambdavirus lambda
3.1.11.3 Mn2+ divalent cation: Mn2+ or Mg2+ required Escherichia coli
3.1.11.3 Mn2+ divalent cation: Mn2+ or Mg2+ required Lambdavirus lambda

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.3 double-stranded DNA + H2O Escherichia coli
-
 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
3.1.11.3 double-stranded DNA + H2O Lambdavirus lambda
-
 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.3 Escherichia coli
-
 Escherichia coli infected with phage lambda only
-
3.1.11.3 Lambdavirus lambda
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.11.3 subfractions upon gradient chromatography: IVa, IVb, phage-lambda induced enzyme Escherichia coli
3.1.11.3 subfractions upon gradient chromatography: IVa, IVb, phage-lambda induced enzyme Lambdavirus lambda
3.1.11.3 to homogeneity Escherichia coli
3.1.11.3 to homogeneity Lambdavirus lambda

Storage Stability

EC Number Storage Stability Organism
3.1.11.3 -20°C, in solution, 1 year, 10% loss of activity Escherichia coli
3.1.11.3 -20°C, in solution, 1 year, 10% loss of activity Lambdavirus lambda
3.1.11.3 0°C, in solution, 2 months, little loss of activity Escherichia coli
3.1.11.3 0°C, in solution, 2 months, little loss of activity Lambdavirus lambda

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.3 double-stranded DNA + H2O
-
 Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
3.1.11.3 double-stranded DNA + H2O
-
 Lambdavirus lambda single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.11.3 60
-
 complete inactivation after 5 min without substrate Escherichia coli
3.1.11.3 60
-
 complete inactivation after 5 min without substrate Lambdavirus lambda

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.11.3 9.2 9.5
-
 Escherichia coli
3.1.11.3 9.2 9.5
-
 Lambdavirus lambda

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.11.3 7 10.2 pH 7.0: 16%, 8.0: 35%, pH 10.0: 35%, 10.2: 8% of maximal activity Escherichia coli
3.1.11.3 7 10.2 pH 7.0: 16%, 8.0: 35%, pH 10.0: 35%, 10.2: 8% of maximal activity Lambdavirus lambda