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Literature summary extracted from

  • Kerr, C.; Sadowski, P.D.
    Gene 6 exonuclease of bacteriophage T7. I. Purification and properties of the enzyme (1972), J. Biol. Chem., 247, 305-310.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.11.3 dithiothreitol required Escherichia coli
3.1.11.3 dithiothreitol required Escherichia phage T7
3.1.11.3 KCl stimulates at 20 mM Escherichia coli
3.1.11.3 KCl stimulates at 20 mM Escherichia phage T7

General Stability

EC Number General Stability Organism
3.1.11.3 stability enhanced by dithiothreitol Escherichia coli
3.1.11.3 stability enhanced by dithiothreitol Escherichia phage T7

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.11.3 Mg2+ required Escherichia coli
3.1.11.3 Mg2+ required Escherichia phage T7
3.1.11.3 Mg2+ optimal concentration 5 mM Escherichia coli
3.1.11.3 Mg2+ optimal concentration 5 mM Escherichia phage T7
3.1.11.3 Mn2+ divalent cation: Mn2+ or Mg2+ required Escherichia coli
3.1.11.3 Mn2+ divalent cation: Mn2+ or Mg2+ required Escherichia phage T7
3.1.11.3 Mn2+ optimal concentration 1 mM Escherichia coli
3.1.11.3 Mn2+ optimal concentration 1 mM Escherichia phage T7

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.3 double-stranded DNA + H2O Escherichia coli
-
 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
3.1.11.3 double-stranded DNA + H2O Escherichia phage T7
-
 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.3 Escherichia coli
-
 Escherichia coli infected with phage lambda only
-
3.1.11.3 Escherichia phage T7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.11.3 partial Escherichia coli
3.1.11.3 partial Escherichia phage T7

Storage Stability

EC Number Storage Stability Organism
3.1.11.3 -20°C, 40% glycerol, 1mg/ml bovine serum albumin, 6 months, 0% loss of activity Escherichia coli
3.1.11.3 -20°C, 40% glycerol, 1mg/ml bovine serum albumin, 6 months, 0% loss of activity Escherichia phage T7

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.3 double-stranded DNA + H2O
-
 Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
3.1.11.3 double-stranded DNA + H2O
-
 Escherichia phage T7 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
3.1.11.3 double-stranded DNA + H2O degradation also at single-stranded breaks within DNA-duplex Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
3.1.11.3 double-stranded DNA + H2O degradation also at single-stranded breaks within DNA-duplex Escherichia phage T7 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
3.1.11.3 double-stranded DNA + H2O degrades double-stranded native DNA Escherichia coli single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?
3.1.11.3 double-stranded DNA + H2O degrades double-stranded native DNA Escherichia phage T7 single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
 ?