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Literature summary extracted from
- The purification of exonuclease I from Escherichia coli by affinity chromatography (1974), J. Biol. Chem., 249, 5379-5381.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.11.1 | EDTA | activity 88% reduced in the presence of Mg2+ | Escherichia coli |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.11.1 | Mg2+ | - |
Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.11.1 | 70000 | - |
2 * 70000, native PAGE | Escherichia coli |
| 3.1.11.1 | 140000 | - |
native PAGE and SDS-PAGE, 85% of activity migrated as dimer, 15% as monomer | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.11.1 | single-stranded polydeoxyribonucleotide + H2O | Escherichia coli | - |
single-stranded oligodeoxynucleotide + nucleoside 5'-monophosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.11.1 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.11.1 | to homogeneitiy by affinity chromatography | Escherichia coli |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.11.1 | cell culture | - |
Escherichia coli | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.11.1 | on ice, 0.02 M Tris-HCl pH 7.4, 1 mM beta-mercaptoethanol, 1 mM EDTA, 10% glycerol, 30% polyethylene glycol, 2 months | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.11.1 | single-stranded polydeoxyribonucleotide + H2O | - |
Escherichia coli | single-stranded oligodeoxynucleotide + nucleoside 5'-monophosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.11.1 | dimer | 2 * 70000, native PAGE | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.11.1 | 9.5 | - |
- |
Escherichia coli |
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Release 2023.1 (January 2023)
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