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Literature summary extracted from
- Phospholipases (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 5, 71-85.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.3 | diethyl ether | substrate: sphingomyelin | Clostridium perfringens |  |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.1.4 | - |
Crotalus adamanteus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.4 | Al3+ | can replace Ca2+, decreased activity | Crotalus adamanteus | |
| 3.1.1.4 | Ba2+ | - |
Crotalus adamanteus | |
| 3.1.1.4 | Ba2+ | - |
Rattus norvegicus | |
| 3.1.1.4 | Ba2+ | - |
Sus scrofa | |
| 3.1.1.4 | Cd2+ | - |
Sus scrofa | |
| 3.1.1.4 | Co2+ | can replace Ca2+, decreased activity | Crotalus adamanteus | |
| 3.1.1.4 | diisopropyl fluorophosphate | - |
Crotalus atrox | |
| 3.1.1.4 | EDTA | - |
Rattus norvegicus | |
| 3.1.1.4 | EDTA | - |
Sus scrofa | |
| 3.1.1.4 | Fe2+ | - |
Crotalus adamanteus | |
| 3.1.1.4 | Fe2+ | - |
Rattus norvegicus | |
| 3.1.1.4 | Pb2+ | - |
Sus scrofa | |
| 3.1.1.4 | Zn2+ | - |
Crotalus adamanteus | |
| 3.1.1.4 | Zn2+ | - |
Crotalus atrox | |
| 3.1.1.4 | Zn2+ | - |
Rattus norvegicus | |
| 3.1.4.3 | 2-mercaptoethanol | substrate: sphingomyelin | Clostridium perfringens | |
| 3.1.4.3 | Ca2+ | substrate: sphingomyelin | Clostridium perfringens | |
| 3.1.4.3 | Cu2+ | - |
Bacillus cereus | |
| 3.1.4.3 | Cu2+ | substrate: sphingomyelin | Clostridium perfringens | |
| 3.1.4.3 | dithiothreitol | - |
Bacillus cereus | |
| 3.1.4.3 | EDTA | - |
Bacillus cereus | |
| 3.1.4.3 | EDTA | substrate: sphingomyelin | Clostridium perfringens | |
| 3.1.4.3 | Fe2+ | substrate: sphingomyelin | Clostridium perfringens | |
| 3.1.4.3 | Zn2+ | substrate: sphingomyelin | Clostridium perfringens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.4 | 8.3 | - |
lecithin | in ether | Crotalus atrox | |
| 3.1.1.4 | 8.5 | - |
lecithin | in chloroform | Crotalus atrox | |
| 3.1.4.3 | 0.8 | - |
sphingomyelin | - |
Clostridium perfringens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.3 | extracellular | - |
Bacillus cereus | - |
- |
| 3.1.4.3 | extracellular | - |
Clostridium perfringens | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.4 | Ca2+ | - |
Rattus norvegicus | |
| 3.1.1.4 | Ca2+ | - |
Sus scrofa | |
| 3.1.1.4 | Ca2+ | - |
Crotalus adamanteus | |
| 3.1.1.4 | Ca2+ | - |
Crotalus atrox | |
| 3.1.1.4 | Cd2+ | can replace Ca2+, decreased activity | Crotalus adamanteus | |
| 3.1.1.4 | Mg2+ | can replace Ca2+, decreased activity | Crotalus adamanteus | |
| 3.1.1.4 | Mn2+ | can replace Ca2+ but enzyme shows decreased activity | Crotalus adamanteus | |
| 3.1.4.3 | Ca2+ | - |
Clostridium perfringens | |
| 3.1.4.3 | Mg2+ | restores ability of Ca2+-inhibited PLC to hydrolyse sphingomyelin | Clostridium perfringens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.3 | phosphatidylcholine + H2O | Bacillus cereus | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | phosphatidylcholine + H2O | Clostridium perfringens | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | phosphatidylcholine + H2O | Clostridium welchii | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | phosphatidylcholine + H2O | Bacillus cereus 7004 | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.1.4.3 | diethyl ether | activating | Clostridium perfringens |
| 3.1.4.3 | mercaptoethanol | inhibitory | Clostridium perfringens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.4 | Crotalus adamanteus | - |
- |
- |
| 3.1.1.4 | Crotalus atrox | - |
- |
- |
| 3.1.1.4 | Rattus norvegicus | - |
- |
- |
| 3.1.1.4 | Sus scrofa | - |
- |
- |
| 3.1.4.3 | Bacillus cereus | - |
- |
- |
| 3.1.4.3 | Bacillus cereus 7004 | - |
- |
- |
| 3.1.4.3 | Clostridium perfringens | - |
strain ATCC 10543 | - |
| 3.1.4.3 | Clostridium welchii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.4 | ammonium sulfate fractionation, column chromatography | Crotalus atrox |
| 3.1.1.4 | column chromatography | Sus scrofa |
| 3.1.1.4 | gel filtration, column chromatography | Crotalus adamanteus |
| 3.1.4.3 | ammonium sulfate precipitation, column chromatography steps | Clostridium perfringens |
| 3.1.4.3 | dialysis, column chromatography, 22fold | Bacillus cereus |
| 3.1.4.3 | protamine precipitation, column chromatography, 23fold | Bacillus cereus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.4 | pancreas | - |
Sus scrofa | - |
| 3.1.1.4 | venom | - |
Crotalus adamanteus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.4 | 3150 | - |
substrate: phosphatidylcholine | Crotalus adamanteus |
| 3.1.1.4 | 3250 | - |
substrate: phosphatidylcholine | Crotalus adamanteus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.1.4 | 4°C, for months without loss of activity | Crotalus adamanteus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.4 | diphosphatidylglycerol + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.1.1.4 | phosphatidic acid + H2O | - |
Sus scrofa | 1-acylglycerophosphate + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidic acid + H2O | - |
Crotalus adamanteus | 1-acylglycerophosphate + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylcholine + H2O | - |
Rattus norvegicus | 1-acylglycerophosphocholine + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylcholine + H2O | - |
Sus scrofa | 1-acylglycerophosphocholine + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylcholine + H2O | - |
Crotalus adamanteus | 1-acylglycerophosphocholine + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylcholine + H2O | - |
Crotalus atrox | 1-acylglycerophosphocholine + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylethanolamine + H2O | - |
Rattus norvegicus | 1-acylglycerophosphorylethanolamine + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylethanolamine + H2O | - |
Sus scrofa | 1-acylglycerophosphorylethanolamine + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylglycerol + H2O | - |
Rattus norvegicus | 1-acylglycerol + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylglycerol + H2O | - |
Sus scrofa | 1-acylglycerol + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylglycerol + H2O | - |
Crotalus adamanteus | 1-acylglycerol + fatty acid | - |
? | |
| 3.1.4.3 | diacylglycerylphosphoryl monomethylethanolamine + H2O | - |
Bacillus cereus | ? | - |
? | |
| 3.1.4.3 | diacylglycerylphosphoryl monomethylethanolamine + H2O | - |
Bacillus cereus 7004 | ? | - |
? | |
| 3.1.4.3 | lysophosphatidylcholine + H2O | - |
Clostridium perfringens | monoacylglycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | phosphatidylcholine + H2O | - |
Bacillus cereus | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | phosphatidylcholine + H2O | - |
Clostridium perfringens | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | phosphatidylcholine + H2O | - |
Clostridium welchii | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | phosphatidylcholine + H2O | - |
Bacillus cereus 7004 | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | phosphatidylethanolamine + H2O | - |
Bacillus cereus | phosphorylethanolamine + diacylglycerol | - |
? | |
| 3.1.4.3 | phosphatidylethanolamine + H2O | - |
Clostridium perfringens | phosphorylethanolamine + diacylglycerol | - |
? | |
| 3.1.4.3 | phosphatidylethanolamine + H2O | - |
Bacillus cereus 7004 | phosphorylethanolamine + diacylglycerol | - |
? | |
| 3.1.4.3 | sphingomyelin + H2O | - |
Bacillus cereus | ceramide + phosphorylcholine | - |
? | |
| 3.1.4.3 | sphingomyelin + H2O | - |
Clostridium perfringens | ceramide + phosphorylcholine | - |
? | |
| 3.1.4.3 | sphingomyelin + H2O | - |
Bacillus cereus 7004 | ceramide + phosphorylcholine | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.4 | 80 | - |
for 30 min., pH 3.0 | Crotalus atrox |
| 3.1.1.4 | 98 | - |
for 5 min., pH 4.0, no loss of activity, for 1 h, pH 4.0, 45% of activity are recovered | Sus scrofa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.4 | 26.7 | - |
phosphatidylcholine | - |
Crotalus adamanteus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.3 | 7.8 | 8.8 | substrate: sphingomyelin | Clostridium perfringens |
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