BRENDA - Enzyme Database

Phospholipases

Hanahan, D.J.; The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ) 5, 71-85 (1971)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
diethyl ether
substrate: sphingomyelin
Clostridium perfringens
Crystallization (Commentary)
EC Number
Crystallization
Organism
3.1.1.4
-
Crotalus adamanteus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
Al3+
can replace Ca2+, decreased activity
Crotalus adamanteus
3.1.1.4
Ba2+
-
Crotalus adamanteus
3.1.1.4
Ba2+
-
Rattus norvegicus
3.1.1.4
Ba2+
-
Sus scrofa
3.1.1.4
Cd2+
-
Sus scrofa
3.1.1.4
Co2+
can replace Ca2+, decreased activity
Crotalus adamanteus
3.1.1.4
diisopropyl fluorophosphate
-
Crotalus atrox
3.1.1.4
EDTA
-
Rattus norvegicus
3.1.1.4
EDTA
-
Sus scrofa
3.1.1.4
Fe2+
-
Crotalus adamanteus
3.1.1.4
Fe2+
-
Rattus norvegicus
3.1.1.4
Pb2+
-
Sus scrofa
3.1.1.4
Zn2+
-
Crotalus adamanteus
3.1.1.4
Zn2+
-
Crotalus atrox
3.1.1.4
Zn2+
-
Rattus norvegicus
3.1.4.3
2-mercaptoethanol
substrate: sphingomyelin
Clostridium perfringens
3.1.4.3
Ca2+
substrate: sphingomyelin
Clostridium perfringens
3.1.4.3
Cu2+
-
Bacillus cereus
3.1.4.3
Cu2+
substrate: sphingomyelin
Clostridium perfringens
3.1.4.3
dithiothreitol
-
Bacillus cereus
3.1.4.3
EDTA
substrate: sphingomyelin
Clostridium perfringens
3.1.4.3
EDTA
-
Bacillus cereus
3.1.4.3
Fe2+
substrate: sphingomyelin
Clostridium perfringens
3.1.4.3
Zn2+
substrate: sphingomyelin
Clostridium perfringens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.4
8.3
-
lecithin
in ether
Crotalus atrox
3.1.1.4
8.5
-
lecithin
in chloroform
Crotalus atrox
3.1.4.3
0.8
-
sphingomyelin
-
Clostridium perfringens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Clostridium perfringens
-
-
3.1.4.3
extracellular
-
Bacillus cereus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Crotalus adamanteus
3.1.1.4
Ca2+
-
Crotalus atrox
3.1.1.4
Ca2+
-
Rattus norvegicus
3.1.1.4
Ca2+
-
Sus scrofa
3.1.1.4
Cd2+
can replace Ca2+, decreased activity
Crotalus adamanteus
3.1.1.4
Mg2+
can replace Ca2+, decreased activity
Crotalus adamanteus
3.1.1.4
Mn2+
can replace Ca2+ but enzyme shows decreased activity
Crotalus adamanteus
3.1.4.3
Ca2+
-
Clostridium perfringens
3.1.4.3
Mg2+
restores ability of Ca2+-inhibited PLC to hydrolyse sphingomyelin
Clostridium perfringens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.3
phosphatidylcholine + H2O
Bacillus cereus
-
1,2-diacylglycerol + phosphorylcholine
-
Bacillus cereus
?
3.1.4.3
phosphatidylcholine + H2O
Clostridium perfringens
-
1,2-diacylglycerol + phosphorylcholine
-
Clostridium perfringens
?
3.1.4.3
phosphatidylcholine + H2O
Clostridium welchii
-
1,2-diacylglycerol + phosphorylcholine
-
Clostridium welchii
?
3.1.4.3
phosphatidylcholine + H2O
Bacillus cereus 7004
-
1,2-diacylglycerol + phosphorylcholine
-
Bacillus cereus 7004
?
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
3.1.4.3
diethyl ether
activating
Clostridium perfringens
3.1.4.3
mercaptoethanol
inhibitory
Clostridium perfringens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.4
Crotalus adamanteus
-
-
-
3.1.1.4
Crotalus atrox
-
-
-
3.1.1.4
Rattus norvegicus
-
-
-
3.1.1.4
Sus scrofa
-
-
-
3.1.4.3
Bacillus cereus
-
-
-
3.1.4.3
Bacillus cereus 7004
-
-
-
3.1.4.3
Clostridium perfringens
-
strain ATCC 10543
-
3.1.4.3
Clostridium welchii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.4
ammonium sulfate fractionation, column chromatography
Crotalus atrox
3.1.1.4
column chromatography
Sus scrofa
3.1.1.4
gel filtration, column chromatography
Crotalus adamanteus
3.1.4.3
ammonium sulfate precipitation, column chromatography steps
Clostridium perfringens
3.1.4.3
dialysis, column chromatography, 22fold; protamine precipitation, column chromatography, 23fold
Bacillus cereus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
pancreas
-
Sus scrofa
-
3.1.1.4
venom
-
Crotalus adamanteus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.1.4
3150
-
substrate: phosphatidylcholine
Crotalus adamanteus
3.1.1.4
3250
-
substrate: phosphatidylcholine
Crotalus adamanteus
Storage Stability
EC Number
Storage Stability
Organism
3.1.1.4
4░C, for months without loss of activity
Crotalus adamanteus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
diphosphatidylglycerol + H2O
-
133931
Rattus norvegicus
?
-
-
-
?
3.1.1.4
phosphatidic acid + H2O
-
133931
Sus scrofa
1-acylglycerophosphate + fatty acid
-
133931
Sus scrofa
?
3.1.1.4
phosphatidic acid + H2O
-
133931
Crotalus adamanteus
1-acylglycerophosphate + fatty acid
-
133931
Crotalus adamanteus
?
3.1.1.4
phosphatidylcholine + H2O
-
133931
Rattus norvegicus
1-acylglycerophosphocholine + fatty acid
-
133931
Rattus norvegicus
?
3.1.1.4
phosphatidylcholine + H2O
-
133931
Sus scrofa
1-acylglycerophosphocholine + fatty acid
-
133931
Sus scrofa
?
3.1.1.4
phosphatidylcholine + H2O
-
133931
Crotalus adamanteus
1-acylglycerophosphocholine + fatty acid
-
133931
Crotalus adamanteus
?
3.1.1.4
phosphatidylcholine + H2O
-
133931
Crotalus atrox
1-acylglycerophosphocholine + fatty acid
-
133931
Crotalus atrox
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133931
Rattus norvegicus
1-acylglycerophosphorylethanolamine + fatty acid
-
133931
Rattus norvegicus
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133931
Sus scrofa
1-acylglycerophosphorylethanolamine + fatty acid
-
133931
Sus scrofa
?
3.1.1.4
phosphatidylglycerol + H2O
-
133931
Rattus norvegicus
1-acylglycerol + fatty acid
-
133931
Rattus norvegicus
?
3.1.1.4
phosphatidylglycerol + H2O
-
133931
Sus scrofa
1-acylglycerol + fatty acid
-
133931
Sus scrofa
?
3.1.1.4
phosphatidylglycerol + H2O
-
133931
Crotalus adamanteus
1-acylglycerol + fatty acid
-
133931
Crotalus adamanteus
?
3.1.4.3
diacylglycerylphosphoryl monomethylethanolamine + H2O
-
133931
Bacillus cereus
?
-
-
-
?
3.1.4.3
diacylglycerylphosphoryl monomethylethanolamine + H2O
-
133931
Bacillus cereus 7004
?
-
-
-
?
3.1.4.3
lysophosphatidylcholine + H2O
-
133931
Clostridium perfringens
monoacylglycerol + phosphorylcholine
-
133931
Clostridium perfringens
?
3.1.4.3
phosphatidylcholine + H2O
-
133931
Bacillus cereus
1,2-diacylglycerol + phosphorylcholine
-
133931
Bacillus cereus
?
3.1.4.3
phosphatidylcholine + H2O
-
133931
Clostridium perfringens
1,2-diacylglycerol + phosphorylcholine
-
133931
Clostridium perfringens
?
3.1.4.3
phosphatidylcholine + H2O
-
133931
Clostridium welchii
1,2-diacylglycerol + phosphorylcholine
-
133931
Clostridium welchii
?
3.1.4.3
phosphatidylcholine + H2O
-
133931
Bacillus cereus 7004
1,2-diacylglycerol + phosphorylcholine
-
133931
Bacillus cereus 7004
?
3.1.4.3
phosphatidylethanolamine + H2O
-
133931
Bacillus cereus
phosphorylethanolamine + diacylglycerol
-
133931
Bacillus cereus
?
3.1.4.3
phosphatidylethanolamine + H2O
-
133931
Clostridium perfringens
phosphorylethanolamine + diacylglycerol
-
133931
Clostridium perfringens
?
3.1.4.3
phosphatidylethanolamine + H2O
-
133931
Bacillus cereus 7004
phosphorylethanolamine + diacylglycerol
-
133931
Bacillus cereus 7004
?
3.1.4.3
sphingomyelin + H2O
-
133931
Bacillus cereus
ceramide + phosphorylcholine
-
133931
Bacillus cereus
?
3.1.4.3
sphingomyelin + H2O
-
133931
Clostridium perfringens
ceramide + phosphorylcholine
-
133931
Clostridium perfringens
?
3.1.4.3
sphingomyelin + H2O
-
133931
Bacillus cereus 7004
ceramide + phosphorylcholine
-
133931
Bacillus cereus 7004
?
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.1.1.4
80
-
for 30 min., pH 3.0
Crotalus atrox
3.1.1.4
98
-
for 5 min., pH 4.0, no loss of activity, for 1 h, pH 4.0, 45% of activity are recovered
Sus scrofa
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.1.4
26.7
-
phosphatidylcholine
-
Crotalus adamanteus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
7.8
8.8
substrate: sphingomyelin
Clostridium perfringens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
diethyl ether
substrate: sphingomyelin
Clostridium perfringens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.1.1.4
-
Crotalus adamanteus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
Al3+
can replace Ca2+, decreased activity
Crotalus adamanteus
3.1.1.4
Ba2+
-
Crotalus adamanteus
3.1.1.4
Ba2+
-
Rattus norvegicus
3.1.1.4
Ba2+
-
Sus scrofa
3.1.1.4
Cd2+
-
Sus scrofa
3.1.1.4
Co2+
can replace Ca2+, decreased activity
Crotalus adamanteus
3.1.1.4
diisopropyl fluorophosphate
-
Crotalus atrox
3.1.1.4
EDTA
-
Rattus norvegicus
3.1.1.4
EDTA
-
Sus scrofa
3.1.1.4
Fe2+
-
Crotalus adamanteus
3.1.1.4
Fe2+
-
Rattus norvegicus
3.1.1.4
Pb2+
-
Sus scrofa
3.1.1.4
Zn2+
-
Crotalus adamanteus
3.1.1.4
Zn2+
-
Crotalus atrox
3.1.1.4
Zn2+
-
Rattus norvegicus
3.1.4.3
2-mercaptoethanol
substrate: sphingomyelin
Clostridium perfringens
3.1.4.3
Ca2+
substrate: sphingomyelin
Clostridium perfringens
3.1.4.3
Cu2+
-
Bacillus cereus
3.1.4.3
Cu2+
substrate: sphingomyelin
Clostridium perfringens
3.1.4.3
dithiothreitol
-
Bacillus cereus
3.1.4.3
EDTA
substrate: sphingomyelin
Clostridium perfringens
3.1.4.3
EDTA
-
Bacillus cereus
3.1.4.3
Fe2+
substrate: sphingomyelin
Clostridium perfringens
3.1.4.3
Zn2+
substrate: sphingomyelin
Clostridium perfringens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.4
8.3
-
lecithin
in ether
Crotalus atrox
3.1.1.4
8.5
-
lecithin
in chloroform
Crotalus atrox
3.1.4.3
0.8
-
sphingomyelin
-
Clostridium perfringens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Clostridium perfringens
-
-
3.1.4.3
extracellular
-
Bacillus cereus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Crotalus adamanteus
3.1.1.4
Ca2+
-
Crotalus atrox
3.1.1.4
Ca2+
-
Rattus norvegicus
3.1.1.4
Ca2+
-
Sus scrofa
3.1.1.4
Cd2+
can replace Ca2+, decreased activity
Crotalus adamanteus
3.1.1.4
Mg2+
can replace Ca2+, decreased activity
Crotalus adamanteus
3.1.1.4
Mn2+
can replace Ca2+ but enzyme shows decreased activity
Crotalus adamanteus
3.1.4.3
Ca2+
-
Clostridium perfringens
3.1.4.3
Mg2+
restores ability of Ca2+-inhibited PLC to hydrolyse sphingomyelin
Clostridium perfringens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.3
phosphatidylcholine + H2O
Bacillus cereus
-
1,2-diacylglycerol + phosphorylcholine
-
Bacillus cereus
?
3.1.4.3
phosphatidylcholine + H2O
Clostridium perfringens
-
1,2-diacylglycerol + phosphorylcholine
-
Clostridium perfringens
?
3.1.4.3
phosphatidylcholine + H2O
Clostridium welchii
-
1,2-diacylglycerol + phosphorylcholine
-
Clostridium welchii
?
3.1.4.3
phosphatidylcholine + H2O
Bacillus cereus 7004
-
1,2-diacylglycerol + phosphorylcholine
-
Bacillus cereus 7004
?
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
3.1.4.3
diethyl ether
activating
Clostridium perfringens
3.1.4.3
mercaptoethanol
inhibitory
Clostridium perfringens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.4
ammonium sulfate fractionation, column chromatography
Crotalus atrox
3.1.1.4
column chromatography
Sus scrofa
3.1.1.4
gel filtration, column chromatography
Crotalus adamanteus
3.1.4.3
ammonium sulfate precipitation, column chromatography steps
Clostridium perfringens
3.1.4.3
dialysis, column chromatography, 22fold; protamine precipitation, column chromatography, 23fold
Bacillus cereus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
pancreas
-
Sus scrofa
-
3.1.1.4
venom
-
Crotalus adamanteus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.1.4
3150
-
substrate: phosphatidylcholine
Crotalus adamanteus
3.1.1.4
3250
-
substrate: phosphatidylcholine
Crotalus adamanteus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.1.4
4░C, for months without loss of activity
Crotalus adamanteus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
diphosphatidylglycerol + H2O
-
133931
Rattus norvegicus
?
-
-
-
?
3.1.1.4
phosphatidic acid + H2O
-
133931
Sus scrofa
1-acylglycerophosphate + fatty acid
-
133931
Sus scrofa
?
3.1.1.4
phosphatidic acid + H2O
-
133931
Crotalus adamanteus
1-acylglycerophosphate + fatty acid
-
133931
Crotalus adamanteus
?
3.1.1.4
phosphatidylcholine + H2O
-
133931
Rattus norvegicus
1-acylglycerophosphocholine + fatty acid
-
133931
Rattus norvegicus
?
3.1.1.4
phosphatidylcholine + H2O
-
133931
Sus scrofa
1-acylglycerophosphocholine + fatty acid
-
133931
Sus scrofa
?
3.1.1.4
phosphatidylcholine + H2O
-
133931
Crotalus adamanteus
1-acylglycerophosphocholine + fatty acid
-
133931
Crotalus adamanteus
?
3.1.1.4
phosphatidylcholine + H2O
-
133931
Crotalus atrox
1-acylglycerophosphocholine + fatty acid
-
133931
Crotalus atrox
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133931
Rattus norvegicus
1-acylglycerophosphorylethanolamine + fatty acid
-
133931
Rattus norvegicus
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133931
Sus scrofa
1-acylglycerophosphorylethanolamine + fatty acid
-
133931
Sus scrofa
?
3.1.1.4
phosphatidylglycerol + H2O
-
133931
Rattus norvegicus
1-acylglycerol + fatty acid
-
133931
Rattus norvegicus
?
3.1.1.4
phosphatidylglycerol + H2O
-
133931
Sus scrofa
1-acylglycerol + fatty acid
-
133931
Sus scrofa
?
3.1.1.4
phosphatidylglycerol + H2O
-
133931
Crotalus adamanteus
1-acylglycerol + fatty acid
-
133931
Crotalus adamanteus
?
3.1.4.3
diacylglycerylphosphoryl monomethylethanolamine + H2O
-
133931
Bacillus cereus
?
-
-
-
?
3.1.4.3
diacylglycerylphosphoryl monomethylethanolamine + H2O
-
133931
Bacillus cereus 7004
?
-
-
-
?
3.1.4.3
lysophosphatidylcholine + H2O
-
133931
Clostridium perfringens
monoacylglycerol + phosphorylcholine
-
133931
Clostridium perfringens
?
3.1.4.3
phosphatidylcholine + H2O
-
133931
Bacillus cereus
1,2-diacylglycerol + phosphorylcholine
-
133931
Bacillus cereus
?
3.1.4.3
phosphatidylcholine + H2O
-
133931
Clostridium perfringens
1,2-diacylglycerol + phosphorylcholine
-
133931
Clostridium perfringens
?
3.1.4.3
phosphatidylcholine + H2O
-
133931
Clostridium welchii
1,2-diacylglycerol + phosphorylcholine
-
133931
Clostridium welchii
?
3.1.4.3
phosphatidylcholine + H2O
-
133931
Bacillus cereus 7004
1,2-diacylglycerol + phosphorylcholine
-
133931
Bacillus cereus 7004
?
3.1.4.3
phosphatidylethanolamine + H2O
-
133931
Bacillus cereus
phosphorylethanolamine + diacylglycerol
-
133931
Bacillus cereus
?
3.1.4.3
phosphatidylethanolamine + H2O
-
133931
Clostridium perfringens
phosphorylethanolamine + diacylglycerol
-
133931
Clostridium perfringens
?
3.1.4.3
phosphatidylethanolamine + H2O
-
133931
Bacillus cereus 7004
phosphorylethanolamine + diacylglycerol
-
133931
Bacillus cereus 7004
?
3.1.4.3
sphingomyelin + H2O
-
133931
Bacillus cereus
ceramide + phosphorylcholine
-
133931
Bacillus cereus
?
3.1.4.3
sphingomyelin + H2O
-
133931
Clostridium perfringens
ceramide + phosphorylcholine
-
133931
Clostridium perfringens
?
3.1.4.3
sphingomyelin + H2O
-
133931
Bacillus cereus 7004
ceramide + phosphorylcholine
-
133931
Bacillus cereus 7004
?
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.1.1.4
80
-
for 30 min., pH 3.0
Crotalus atrox
3.1.1.4
98
-
for 5 min., pH 4.0, no loss of activity, for 1 h, pH 4.0, 45% of activity are recovered
Sus scrofa
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.1.4
26.7
-
phosphatidylcholine
-
Crotalus adamanteus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
7.8
8.8
substrate: sphingomyelin
Clostridium perfringens