BRENDA - Enzyme Database

Purification and some properties of rat spleen phospholipase A

Rahman, Y.E.; Cerny, E.A.; Peraino, C.; Biochim. Biophys. Acta 321, 526-535 (1973)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
Cu2+
-
Rattus norvegicus
3.1.1.4
deoxycholate
substrate: phosphatidylcholine
Rattus norvegicus
3.1.1.4
EDTA
-
Rattus norvegicus
3.1.1.4
EDTA
-
Sus scrofa
3.1.1.4
Fe2+
-
Rattus norvegicus
3.1.1.4
Hg2+
-
Rattus norvegicus
3.1.1.4
Mg2+
-
Rattus norvegicus
3.1.1.4
Triton X-100
-
Rattus norvegicus
3.1.1.4
Zn2+
-
Rattus norvegicus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Rattus norvegicus
3.1.1.4
Ca2+
-
Sus scrofa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
15000
-
gel filtration, gel electrophoresis, SDS-PAGE
Rattus norvegicus
3.1.1.4
15000
-
-
Sus scrofa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Rattus norvegicus
-
?
-
-
-
3.1.1.4
phospholipids + H2O
Sus scrofa
-
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.4
Rattus norvegicus
-
-
-
3.1.1.4
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.4
column chromatography
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
pancreas
-
Sus scrofa
-
3.1.1.4
spleen
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.4
5000
-
substrate: phosphatidylethanolamine
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
-
133928
Rattus norvegicus
1-palmitoyl-glycerophosphorylethanolamine + linolic acid
-
-
-
?
3.1.1.4
phosphatidylcholine + H2O
-
133928
Rattus norvegicus
1-acylglycerophosphocholine + fatty acid
-
133928
Rattus norvegicus
?
3.1.1.4
phosphatidylcholine + H2O
-
133928
Sus scrofa
1-acylglycerophosphocholine + fatty acid
-
133928
Sus scrofa
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133928
Rattus norvegicus
1-acylglycerophosphorylethanolamine + fatty acid
-
133928
Rattus norvegicus
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133928
Sus scrofa
1-acylglycerophosphorylethanolamine + fatty acid
-
133928
Sus scrofa
?
3.1.1.4
phospholipids + H2O
-
133928
Rattus norvegicus
?
-
-
-
-
3.1.1.4
phospholipids + H2O
-
133928
Sus scrofa
?
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.4
60
-
for 10 min at pH 5.0 no loss of activity, at pH 7.0 50% of activity remains, pH 9.0 leads to complete loss of activity
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
7
-
-
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.4
6
9
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
Cu2+
-
Rattus norvegicus
3.1.1.4
deoxycholate
substrate: phosphatidylcholine
Rattus norvegicus
3.1.1.4
EDTA
-
Rattus norvegicus
3.1.1.4
EDTA
-
Sus scrofa
3.1.1.4
Fe2+
-
Rattus norvegicus
3.1.1.4
Hg2+
-
Rattus norvegicus
3.1.1.4
Mg2+
-
Rattus norvegicus
3.1.1.4
Triton X-100
-
Rattus norvegicus
3.1.1.4
Zn2+
-
Rattus norvegicus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Rattus norvegicus
3.1.1.4
Ca2+
-
Sus scrofa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
15000
-
gel filtration, gel electrophoresis, SDS-PAGE
Rattus norvegicus
3.1.1.4
15000
-
-
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
phospholipids + H2O
Rattus norvegicus
-
?
-
-
-
3.1.1.4
phospholipids + H2O
Sus scrofa
-
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.4
column chromatography
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
pancreas
-
Sus scrofa
-
3.1.1.4
spleen
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.4
5000
-
substrate: phosphatidylethanolamine
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
-
133928
Rattus norvegicus
1-palmitoyl-glycerophosphorylethanolamine + linolic acid
-
-
-
?
3.1.1.4
phosphatidylcholine + H2O
-
133928
Rattus norvegicus
1-acylglycerophosphocholine + fatty acid
-
133928
Rattus norvegicus
?
3.1.1.4
phosphatidylcholine + H2O
-
133928
Sus scrofa
1-acylglycerophosphocholine + fatty acid
-
133928
Sus scrofa
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133928
Rattus norvegicus
1-acylglycerophosphorylethanolamine + fatty acid
-
133928
Rattus norvegicus
?
3.1.1.4
phosphatidylethanolamine + H2O
-
133928
Sus scrofa
1-acylglycerophosphorylethanolamine + fatty acid
-
133928
Sus scrofa
?
3.1.1.4
phospholipids + H2O
-
133928
Rattus norvegicus
?
-
-
-
-
3.1.1.4
phospholipids + H2O
-
133928
Sus scrofa
?
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.4
60
-
for 10 min at pH 5.0 no loss of activity, at pH 7.0 50% of activity remains, pH 9.0 leads to complete loss of activity
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
7
-
-
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.4
6
9
-
Rattus norvegicus